View Item 
  •   e_Buah Home
  • INVESTIGACIÓN
  • DEPARTAMENTOS
  • Química Analítica, Química Física e Ingeniería Química
  • Unidad docente Química Analítica e Ingeniería Quimica
  • QUANING - Artículos
  • View Item
  • INVESTIGACIÓN
  • DEPARTAMENTOS
  • Química Analítica, Química Física e Ingeniería Química
  • Unidad docente Química Analítica e Ingeniería Quimica
  • QUANING - Artículos
  • View Item
  • Biblioteca
    • English
    • español
JavaScript is disabled for your browser. Some features of this site may not work without it.

Development of an in-capillary derivatization method by CE for the determination of chiral amino acids in dietary supplements and wines

Show full item record
RefworksUtilizar EndNote Import
Authors
Martínez Girón, Ana BelénUniversity of Alcalá Author; García Ruiz, CarmenUniversity of Alcalá Author; Crego Navazo, Antonio LuisUniversity of Alcalá Author; Marina Alegre, María LuisaUniversity of Alcalá Author
Identifiers
Permanent link (URI): http://hdl.handle.net/10017/3888
DOI: 10.1002/elps.200800481
ISSN: 0173-0835
Publisher
WILEY-VCH
Date
2009
Affiliation
Universidad de Alcalá. Departamento de Química Analítica e Ingeniería Química
Funders
The authors thank the Ministry of Education and Science (Spain) and the Comunidad Autónoma de Madrid (Spain) for research projects CTQ2006-03849/BQU and S-0505/AGR- 0312, respectively. Carmen Garcı´a-Ruiz thanks the Ministry of Science and Technology (Spain) for the Ramon y Cajal program (RYC-2003-001). A.B.M.-G. thanks the University of Alcala for her pre-doctoral contract.
Bibliographic citation
Electrophoresis, 2009, v. 30, n. 4, p. 696-704
Keywords
Amino acid
6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate
CE
Chiral method
In-capillary derivatization
Project
CTQ2006-03849/BQU (Ministerio de Educación y Ciencia)
S0505/AGR-0312 (Comunidad de Madrid)
RYC2003-001 (Ministerio de Ciencia y Tecnología)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Publisher's version
http://dx.doi.org/10.1002/elps.200800481
Rights
(c) WILEY-VCH, 2009
Access rights
info:eu-repo/semantics/openAccess
Share
 
Abstract
A fast in-capillary derivatization method by CE with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate was developed for the first time for the determination of amino acid enantiomers (arginine, lysine, and ornithine) in dietary supplements and wines. Because of the initial current problems due to the formation of precipitates into the capillary during the derivatization reaction, a washing step with an organic solvent as DMSO between injections was necessary. Different approaches were also investigated to enhance the sensitivity of detection. A derivatization procedure, where plugs of ACN, derivatizing agent (10mM 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate), and sample in borate (1:1 v/v) were injected in tandem (2, 3, and 6 s, respectively, at 50 mbar), was selected because it enabled to obtain the most sensitive and reproducible results. Appropriate analytical characteristics (linearity, LOD and LOQ, precision, absence of matrix interferences, and accuracy) were obtained for this method. Finally, the optimized method was successfully applied to the determination of the enantiomers of arginine, lysine, and ornithine in food samples of different complexities (dietary supplements and wines).
Files in this item
FilesSizeFormat
View
Orn_in capillary_Electrophoresis ...328.9KbPDF
FilesSizeFormat
View
Orn_in capillary_Electrophoresis ...328.9KbPDF
Collections
  • QUANING - Artículos [250]

Contact Us | Send Feedback | About DSpace
¡CSS Válido!@mire NV
¡CSS Válido!@mire NV
 

 

Browse

All of e_BuahCommunities y CollectionsIssue DateAuthorsTitlesSubjectsIn this CollectionIssue DateAuthorsTitlesSubjects

My Account

My e_BuahCreate account

Help

What is e-Buah?Guide e_BuahGuide autoarchiveFAQContact us

Statistics

View Usage Statistics

Information

Open Science. Open accessOpen access PolicyPublishing permissionsCopyrightResearch datae-cienciaDatos RepositoryPlan de Gestión de Datos

Los contenidos se difunden en


Contact Us | Send Feedback | About DSpace
¡CSS Válido!@mire NV
¡CSS Válido!@mire NV