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dc.contributor.authorMartínez Girón, Ana Belén 
dc.contributor.authorGarcía Ruiz, Carmen 
dc.contributor.authorCrego Navazo, Antonio Luis 
dc.contributor.authorMarina Alegre, María Luisa
dc.identifier.bibliographicCitationElectrophoresis, 2009, v. 30, n. 4, p. 696-704en
dc.description.abstractA fast in-capillary derivatization method by CE with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate was developed for the first time for the determination of amino acid enantiomers (arginine, lysine, and ornithine) in dietary supplements and wines. Because of the initial current problems due to the formation of precipitates into the capillary during the derivatization reaction, a washing step with an organic solvent as DMSO between injections was necessary. Different approaches were also investigated to enhance the sensitivity of detection. A derivatization procedure, where plugs of ACN, derivatizing agent (10mM 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate), and sample in borate (1:1 v/v) were injected in tandem (2, 3, and 6 s, respectively, at 50 mbar), was selected because it enabled to obtain the most sensitive and reproducible results. Appropriate analytical characteristics (linearity, LOD and LOQ, precision, absence of matrix interferences, and accuracy) were obtained for this method. Finally, the optimized method was successfully applied to the determination of the enantiomers of arginine, lysine, and ornithine in food samples of different complexities (dietary supplements and wines).en
dc.description.sponsorshipThe authors thank the Ministry of Education and Science (Spain) and the Comunidad Autónoma de Madrid (Spain) for research projects CTQ2006-03849/BQU and S-0505/AGR- 0312, respectively. Carmen Garcı´a-Ruiz thanks the Ministry of Science and Technology (Spain) for the Ramon y Cajal program (RYC-2003-001). A.B.M.-G. thanks the University of Alcala for her pre-doctoral contract.en
dc.rights(c) WILEY-VCH, 2009en
dc.subjectAmino aciden
dc.subject6-Aminoquinolyl-N-hydroxysuccinimidyl carbamateen
dc.subjectChiral methoden
dc.subjectIn-capillary derivatizationen
dc.titleDevelopment of an in-capillary derivatization method by CE for the determination of chiral amino acids in dietary supplements and winesen
dc.subject.ecienciaQuímica analítica e industriales_ES
dc.subject.ecienciaChemistry, analytic and technicalen
dc.contributor.affiliationUniversidad de Alcalá. Departamento de Química Analítica e Ingeniería Química
dc.relation.projectIDinfo:eu-repo/grantAgreement/MEC//CTQ2006-03849/ES/Nuevas Contribuciones para el Desarrollo de Metodologías Analíticas de Alta Sensibilidad y Resolución Mediante Técnicas Microseparativas/es_ES
dc.relation.projectIDS0505/AGR-0312 (Comunidad de Madrid)

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