RT info:eu-repo/semantics/article
T1 Development of an in-capillary derivatization method by CE for the determination of chiral amino acids in dietary supplements and wines
A1 Martínez Girón, Ana Belén
A1 García Ruiz, Carmen
A1 Crego Navazo, Antonio Luis
A1 Marina Alegre, María Luisa
K1 Amino acid
K1 6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate
K1 CE
K1 Chiral method
K1 In-capillary derivatization
K1 Ciencia
K1 Química analítica e industrial
K1 Science
K1 Chemistry, analytic and technical
AB A fast in-capillary derivatization method by CE with 6-aminoquinolyl-N-hydroxysuccinimidylcarbamate was developed for the first time for the determination ofamino acid enantiomers (arginine, lysine, and ornithine) in dietary supplements andwines. Because of the initial current problems due to the formation of precipitates intothe capillary during the derivatization reaction, a washing step with an organic solvent asDMSO between injections was necessary. Different approaches were also investigated toenhance the sensitivity of detection. A derivatization procedure, where plugs of ACN,derivatizing agent (10mM 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate), andsample in borate (1:1 v/v) were injected in tandem (2, 3, and 6 s, respectively, at 50 mbar),was selected because it enabled to obtain the most sensitive and reproducible results.Appropriate analytical characteristics (linearity, LOD and LOQ, precision, absence ofmatrix interferences, and accuracy) were obtained for this method. Finally, the optimizedmethod was successfully applied to the determination of the enantiomers of arginine,lysine, and ornithine in food samples of different complexities (dietary supplements andwines).
PB WILEY-VCH
SN 0173-0835
YR 2009
FD 2009
LK http://hdl.handle.net/10017/3888
UL http://hdl.handle.net/10017/3888
LA eng
NO The authors thank the Ministry of Education and Science (Spain) and the Comunidad Autónoma de Madrid (Spain) for research projects CTQ2006-03849/BQU and S-0505/AGR- 0312, respectively. Carmen Garcı´a-Ruiz thanks the Ministry of Science and Technology (Spain) for the Ramon y Cajal program (RYC-2003-001). A.B.M.-G. thanks the University of Alcala for her pre-doctoral contract.
DS MINDS@UW
RD 26-abr-2024