Plasma glycocalyx pattern: a mirror of endothelial damage in chronic kidney disease
Authors
Alique Aguilar, Matilde; Valera, Gemma; Figuer Rubio, Andrea; Caro, Jara; Yuste, Claudia; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/60733DOI: 10.1093/ckj/sfad051
ISSN: 2048-8513
Date
2023-03-20Bibliographic citation
Clinical Kidney Journal, 2023, v. 16, n. 8, p. 1278-1287
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
© The Authors
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
Background. Endothelial damage and cardiovascular disease complicate chronic kidney disease (CKD). The increased
atherogenicity observed in patients with CKD can be linked to microinflammation and endothelial damage. Circulating
endothelial glycocalyx degradation products, such as perlecan and decorin, tend to be elevated in CKD. We aimed to
explore the association between the plasma perlecan and decorin levels and this pro-inflammatory and atherogenic
state by studying monocyte subpopulations and intracellular adhesion molecule (ICAM)-1 expression in patients with
CKD.
Methods. We studied 17 healthy controls, 23 patients with advanced CKD, 25 patients on haemodialysis, 23 patients on
peritoneal dialysis and 20 patients who underwent kidney transplantation. Perlecan and decorin levels were evaluated
using enzyme-linked immunosorbent assays, and the monocyte phenotype was analysed using direct
immunofluorescence and flow cytometry.
Results. The plasma perlecan levels were higher in patients with CKD than in the healthy controls. These levels were
associated with a higher prevalence of ICAM-1+ monocytes. Conversely, patients with advanced CKD (pre-dialysis) had
higher plasma decorin levels, which were associated with a reduced ICAM-1 expression per monocyte.
Conclusions. Elevated perlecan levels in CKD may be associated with a higher prevalence of ICAM-1+ monocytes and a
pro-inflammatory phenotype. Elevated decorin levels may act as a negative regulator of ICAM-1 expression in
monocytes. Therefore, perlecan and decorin may be related to inflammation and monocyte activation in CKD and may
act as potential markers of endothelial damage.
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