Toward early diagnosis of late-onset sepsis in preterm neonates: Dual magnetoimmunosensor for simultaneous procalcitonin and C-reactive protein determination in diagnosed clinical samples
Authors
Molinero Fernández, Águeda; Moreno Guzmán, María; Arruza Gómez, Luis; López Gil, Miguel Ángel; Escarpa Miguel, Jesús AlbertoIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/60032DOI: 10.1021/acssensors.9b00890
ISSN: 2379-3694
Date
2019-07-15Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaFunders
Ministerio de Economía y Competitividad
Comunidad Autónoma de Madrid
Fundación “La Caixa”
Bibliographic citation
ACS Sensors, 2019, v. 4, n. 8, p. 2117-2123
Keywords
procalcitonin
c-reactive protein
sepsis biomarkers
dual magnetoimmunosensor
electrochemical detection
Project
info:eu-repo/grantAgreement/MINECO//CTQ201786441-C2-1-R/ES/
info:eu-repo/grantAgreement/CAM/TRANSNANOAVANSENS/S2018%2FNMT-4349/ES/
info:eu-repo/grantAgreement/FLC//La Caixa Impulse/CI017-00038/ES/
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/aceptedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© ACS
Access rights
info:eu-repo/semantics/openAccess
Abstract
Early diagnosis of sepsis, combining blood cultures and inflammation biomarkers, continues to be a challenge, especially in very low birth weight (VLBW) infants because of limited availability of blood samples. Traditional diagnostic procedures are cumbersome, not fast enough, and require relatively large volumes of sample. Empiric use of antibiotics, before diagnostic confirmation, is required to decrease mortality, leading to potential antibiotic resistance and side effects in VLBW infants. To solve such a serious problem, a dual magnetoimmunosensor is proposed for simultaneous assessment of two of the most important sepsis biomarkers: procalcitonin (PCT for early phase) and Creactive protein (CRP for late phase). This ?sample-to-result? approach exhibited excellent sensitivity, selectivity, precision, and stability using low sample volumes (<30 ?L) and under 20 min of total assay. The analytical usefulness of the approach was demonstrated by analyzing clinically relevant samples of preterm neonates with suspicion of sepsis.
Files in this item
Files | Size | Format |
|
---|---|---|---|
Toward_Molinero_ACSSensors_2019.pdf | 903.3Kb |
|
Files | Size | Format |
|
---|---|---|---|
Toward_Molinero_ACSSensors_2019.pdf | 903.3Kb |
|
Collections
- QUANING - Artículos [367]