Ultrastructural Study of Acanthamoeba polyphaga Trophozoites and Cysts Treated In Vitro with Cationic Carbosilane Dendrimers
Authors
Heredero Bermejo, Irene; Martín Pérez, Tania; Copa Patiño, José Luis; Gómez Ramírez, Rafael; Mata de la Mata, Francisco Javier de la; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/59730DOI: 10.3390/pharmaceutics12060565
ISSN: 1999-4923
Date
2020-06-18Affiliation
Universidad de Alcalá. Departamento de Teoría de la Señal y Comunicaciones; Universidad de Alcalá. Departamento de Biomedicina y Biotecnología; Universidad de Alcalá. Departamento de Química Orgánica y Química InorgánicaFunders
Ministerio de Economía y Competitividad
Comunidad de Madrid
Junta de Comunidades de Castilla-La Mancha
Bibliographic citation
Pharmaceutics, 2020, v. 12, n. 6, p. 565-578
Keywords
Acanthamoeba
cysts
trophozoites
dendrimers
scanning electron microscopy
transmission electron microscopy
Project
info:eu-repo/grantAgreement/MINECO//CTQ2017-86224-P/ES/
info:eu-repo/grantAgreement/CAM//B2017%2FBMD-3733/ES/
info:eu-repo/grantAgreement/CAM//B2017%2FBMD-3703/ES/
info:eu-repo/grantAgreement/JCCM//SBPLY%2F17/%2F80501%2F000358
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
© The Authors
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
Cationic carbosilane dendrimers are branched molecules with antimicrobial properties. Their activity has been tested againstAcanthamoeba polyphaga, a causative agent ofAcanthamoebakeratitis, a severe ocular disease in humans.A. polyphagatrophozoites and cysts were exposed to different noncytotoxic cationic carbosilane dendrimers with proven antiamoebic activity. The effects of treatment on cell surface and cell ultrastructure were examined by scanning and transmission electron microscopy, respectively. Two of the dendrimers tested induced dramatic alterations of cellular ultrastructure in both trophozoites and cysts, including vacuolization, depletion of cytoplasmic contents, and reduced cell size. Additionally, we observed severe alterations of the plasma membrane with membrane blebbing in trophozoites and disruption in cysts. These alterations were also observed with chlorhexidine, a drug used for treatment ofAcanthamoebakeratitis. Our results support that these compounds may target membranes, and their action is critical for parasite integrity.
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