In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida albicans Biofilms
Authors
Heredero Bermejo, Irene; Gómez Casanova, Natalia; Quintana Sánchez, Sara; Soliveri de Carranza, Juan; Mata de la Mata, Francisco Javier de la; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/59698DOI: 10.3390/pharmaceutics12100918
ISSN: 1999-4923
Date
2020-09-21Affiliation
Universidad de Alcalá. Departamento de Biomedicina y Biotecnología; Universidad de Alcalá. Departamento de Química Orgánica y Química InorgánicaFunders
Ministerio de Economía, Industria y Competitividad
Comunidad de Madrid
Universidad de Alcalá
Bibliographic citation
Pharmaceutics, 2020, v. 12, n. 10 (918), p. 1-17
Keywords
Candida
antifungal drugs
Candida albicans
biofilm
biocides
carbosilane dendrimers
cytotoxicity
scanning electron microscopy
Project
info:eu-repo/grantAgreement/MINECO//TQ2017-86224-P/ES/
info:eu-repo/grantAgreement/CAM//B2017%2FBMD-3703/ES/
info:eu-repo/grantAgreement/UAH//CCG19%2FCCS-022/ES/
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
© The Authors
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
Candida spp. are one of the most common fungal pathogens. Biofilms formed by Candida albicans offer resistance mechanisms against most antifungal agents. Therefore, development of new molecules effective against these microorganisms, alone or in combination with antifungal drugs, is extremely necessary. In the present work, we carried out a screening process of different cationic carbosilane dendritic molecules against C. albicans. In vitro activity against biofilm formation and biofilms was tested in both Coleccion Espanola de Cultivos Tipo (CECT) 1002 and clinical C. albicans strains. Cytotoxicity was studied in human cell lines, and biofilm alterations were observed by scanning electron microscopy (SEM). Antifungal activity of the carbosilane dendritic molecules was assessed by monitoring cell viability using both established and novel cell viability assays. One out of 14 dendritic molecules tested, named BDSQ024, showed the highest activity with a minimum biofilm inhibitory concentration (MBIC) for biofilm formation and a minimum biofilm damaging concentration (MBDC) for existing biofilm of 16-32 and 16 mg/L, respectively. Synergy with amphotericin (AmB) and caspofungin (CSF) at non-cytotoxic concentrations was found. Therefore, dendritic compounds are exciting new antifungals effective at preventing Candida biofilm formation and represent a potential novel therapeutic agent for treatment of C. albicans infection in combination with existing clinical antifungals.
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