Anticancer Activity of Dendriplexes against Advanced Prostate Cancer from Protumoral Peptides and Cationic Carbosilane Dendrimers
Authors
Sánchez Milla, María; Muñoz Moreno, Laura; Sánchez-Nieves Fernández, Javier; Maly, Marek; Gómez Ramírez, Rafael; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/59674DOI: 10.1021/acs.biomac.8b01632
ISSN: 1525-7797
Date
2019-01-22Affiliation
Universidad de Alcalá. Departamento de Biología de Sistemas; Universidad de Alcalá. Departamento de Química Orgánica y Química InorgánicaFunders
Universidad de Alcalá
Ministerio de Economía, Industria y Competitividad
Comunidad de Madrid
Instituto Ramón y Cajal de Investigación Sanitaria
Bibliographic citation
Biomacromolecules, 2019, v. 20, n. 3, p. 1224-1234
Project
info:eu-repo/grantAgreement/MINECO//CTQ2017-86224-P/ES/
info:eu-repo/grantAgreement/CAM//B2017%2FBMD-3703/ES/
info:eu-repo/grantAgreement/CAM//CCGP2017-EXP%2F023/ES/
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/aceptedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© ACS
Access rights
info:eu-repo/semantics/openAccess
Abstract
The interaction of neuropeptides, vasoactive intestinal peptide (VIP), or growth hormone-releasing hormone (GHRH), with a cationic carbosilane dendrimer forms dendriplexes with antitumoral behavior in advanced prostate cancer cells PC3. At the concentrations used for dendriplexes formation, the free peptides were protumoral and prometastatic in advanced prostate cancer, while dendrimer only showed low cytotoxicity, but did not avoid the metastatic behavior of PC3 cells. However, these nanoplexes favored also cell adhesion and avoided cell migration. Also, the dendriplexes were not toxic for no tumoral prostate cells (RPWE-1) or fibroblasts. The use of labeled GHRH peptide (rhodamine labeled) and a dendrimer (fluorescein labeled) allowed us to observe that both systems reach the intracellular milieu after dendriplex formation. The treatment of PC3 cells with the nanoplexes reduced expression of vascular endothelial growth factor (VEGF) and cyclic adenosine monophosphate (CAMP). Molecular modeling analysis highlights the important contribution of the carbosilane framework in the stabilization of the dendriplex, since dendrimer interacts with a peptide region where hydrophobic amino acids are presented.
Files in this item
Files | Size | Format |
|
---|---|---|---|
Anticancer_Sanchez_Biomacromol ... | 1.669Mb |
|
Files | Size | Format |
|
---|---|---|---|
Anticancer_Sanchez_Biomacromol ... | 1.669Mb |
|
Collections
- QUIMINOR - Artículos [238]