Thermo-responsive antimicrobial hydrogel for the in-situ coating of mesh materials for hernia repair
Authors
Pérez Köhler, Bárbara; Pascual González, María Gemma; Benito Martínez, Selma; García-Moreno Nisa, Francisca; Bellón Caneiro, Juan Manuel; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/59221DOI: 10.3390/polym12061245
ISSN: 2073-4360
Date
2020Affiliation
Universidad de Alcalá. Departamento de Cirugía, Ciencias Médicas y Sociales; Universidad de Alcalá. Departamento de Medicina y Especialidades MédicasFunders
info:eu-repo/grantAgreement/European Hernia Society/2019
info:eu-repo/grantAgreement/MICINN //SAF2017-89481-P
info:eu-repo/grantAgreement/MICINN //SAF2017-89481-P
Bibliographic citation
Polymers, 2020, v. 12, n. 1245, p. 1-15
Keywords
Hernia
Hyaluronic acid
Infection Prophylaxis
Mesh coating
Polypropylene
Rifampicin
Staphylococcus aureus
Thermo-responsive hydrogel
Project
info:eu-repo/grantAgreement/European Hernia Society/2019
info:eu-repo/grantAgreement/MICINN //SAF2017-89481-P
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution 4.0 International (CC BY 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
The prophylactic coating of prosthetic mesh materials for hernia repair with antimicrobial compounds is commonly performed before implantation of the mesh in the abdominal wall. We propose a novel alternative, which is a rifampicin-loaded thermo-responsive hydrogel formulation, to be applied on the mesh after its implantation. This formulation becomes a gel in-situ once reached body temperature, allowing an optimal coating of the mesh along with the surrounding tissues. In vitro, the hydrogel cytotoxicity was assessed using rabbit fibroblasts and antimicrobial e_cacy was determined against Staphylococcus aureus. An in vivo rabbit model of hernia repair was performed; implanted polypropylene meshes (5 x 2 cm) were challenged with S. aureus (106 CFU), for two study groups?unloaded (n = 4) and 0.1 mg/cm2 rifampicin-loaded hydrogel (n = 8). In vitro, antibacterial activity of the hydrogel lasted for 5 days, without sign of cytotoxicity. Fourteen days after implantation, meshes coated with drug-free hydrogel developed a strong infection and resulted in poor tissue integration. Coating meshes with the rifampicin-loaded hydrogel fully prevented implant infection and permitted an optimal tissue integration. Due to its great performance, this, degradable, thermo-responsive antimicrobial hydrogel could potentially be a strong prophylactic armamentarium to be combined with prosthesis in the surgical field.
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thermo_perez_POLYMERS_2020.pdf | 3.441Mb |
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thermo_perez_POLYMERS_2020.pdf | 3.441Mb |
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