Lipid-coated ruthenium dendrimer conjugated with doxorubicin in anti-cancer drug delivery: Introducing protocols
Authors
Michlewska , Silwia; Garaiova , Zuzana; Subjakova , Veronica; Holota, Marcin; Kubczak, Malgorzata; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/57732DOI: https://doi.org/10.1016/j.colsurfb.2023.113371
ISSN: 0927-7765
Date
2023-05-24Bibliographic citation
Colloids and Surfaces B: Biointerfaces, 2023, v. 227, n. 113371
Keywords
Liposomes
Doxorubicin
Dendrimers
Nanocarriers
Drug delivery
Nanotechnology
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
© The authors
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
One of the major limitations for the treatment of many diseases is an inability of drugs to cross the cell membrane barrier. Different kinds of carriers are being investigated to improve drug bioavailability. Among them, lipid or polymer-based systems are of special interest due to their biocompatibility. In our study, we combined dendritic and liposomal carriers and analysed the biochemical and biophysical properties of these formulations. Two preparation methods of Liposomal Locked-in Dendrimers (LLDs) systems have been established and compared. Carbosilane ruthenium metallodendrimer was complexed with an anti-cancer drug (doxorubicin) and locked in a liposomal structure, using both techniques. The LLDs systems formed by hydrophilic locking had more efficient transfection profiles and interacted with the erythrocyte membrane better than systems using the hydrophobic method. The results indicate these systems have improved transfection properties when compared to noncomplexed components. The coating of dendrimers with lipids significantly reduced their hemotoxicity and cytotoxicity. The nanometric size, low polydispersity index and reduced positive zeta potential of such complexes made them attractive for future application in drug delivery. The formulations prepared by the hydrophobic locking protocol were not effective and will not be considered furthermore as prospective drug delivery systems. In contrast, the formulations formed by the hydrophilic loading method have shown promising results where the cytotoxicity of LLD systems with doxorubicin was more effective against cancer than normal cells.
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Lipid_Michlewska_CollSurfB_2023.pdf | 6.190Mb |
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Lipid_Michlewska_CollSurfB_2023.pdf | 6.190Mb |
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