Effect of meso vs macro-size of hierarchical porous silica on the adsorption and activity of immobilized beta-galactosidase
Authors
Pavel, Ileana A.; Fontaínhas Prazeres, Sofía; Montalvo García, Gemma; García Ruiz, Carmen; Nicolas, Vicent; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/48155DOI: 10.1021/acs.langmuir.7b00134
ISSN: 0743-7463
Date
2017-03-16Affiliation
Universidad de Alcalá. Departamento de Química Analítica Química Física e Ingeniería QuímicaFunders
7º Programa Marco de la UE FP7/2007-2013/, Programa People.
Bibliographic citation
Langmuir : the ACS journal of surfaces and colloids, 2017, v. 33, n. 13, p. 3333-3340
Project
FP7-PEOPLE-2013-ITN-606713 (7º Programa Marco de la UE FP7/2007-2013/, Programa People)
"Biopolymer Based Food Delivery Systems (BIBAFOODS)"
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© ACS Publications, 2017
Access rights
info:eu-repo/semantics/openAccess
Abstract
beta-Galactosidase (beta-Gal) is one of the most important enzymes used in milk processing for improving their nutritional quality and digestibility. Herein, beta-Gal has been entrapped into a meso-macroporous material (average pore size 9 and 200 nm, respectively) prepared by a sol&-gel method from a silica precursor and a dispersion of solid lipid nanoparticles in a micelle phase. The physisorption of the enzyme depends on the concentration of the feed solution and on the pore size of the support. The enzyme is preferentially adsorbed either in mesopores or in macropores, depending on its initial concentration. Moreover, this selective adsorption, arising from the oligomeric complexation of the enzyme (monomer/dimer/tetramer), has an effect on the catalytic activity of the material. Indeed, the enzyme encapsulated in macropores is more active than the enzyme immobilized in mesopores. Designed materials containing &;946#-Gal are of particular interest for food applications and potentially extended to bioconversion, bioremediation, or biosensing when coupling the designed support with other enzymes.
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