Separation and identification of peptides in hydrolysed protein extracts from edible macroalgae by HPLC-ESI-QTOF/MS
Authors
Pérez Miguez, Raquel; Plaza del Moral, Merichel; Castro Puyana, María; Marina Alegre, María LuisaIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/48128DOI: https://doi.org/10.1016/j.algal.2019.101465
ISSN: 2211-9264
Date
2019-05Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaFunders
Comunidad de Madrid
Bibliographic citation
Algal Research-Biomass Biofuels and Bioproducts, 2019, v. 39, n. 101465
Keywords
Peptides
Reversed-phase
Liquid chromatography-tandem mass spectrometry
Macroalgae
Project
info:eu-repo/grantAgreement/CAM//S2018%2FBAA-4393/ES/ESTRATEGIAS INTEGRADAS PARA LA MEJORA DE LA CALIDAD, LA SEGURIDAD Y LA FUNCIONALIDAD DE LOS ALIMENTOS: HACIA UNA ALIMENTACIÓN SALUDABLE/AVANSECAL-II
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
© Elsevier
Access rights
info:eu-repo/semantics/openAccess
Abstract
Macroalgae contain significant amounts of high-quality proteins which, because of their structural diversity, contain a range of yet undiscovered peptides within their primary structures. In this work, an analytical methodology was developed for the separation and identification of peptides present in protein hydrolysates from three different edible macroalgae used for human consumption (Saccharina latissima (brown macroalga), Codium spp. (green macroalga), and Mastocarpus stellatus (red macroalga)). The extraction of aqueous and alkaline soluble proteins was carried out followed by their precipitation with HCl or acetone. The protein extracts obtained were submitted to enzymatic digestion with alcalase and subsequently analyzed by reversed-phase high-performance liquid chromatography-quadrupole-time-of flight mass spectrometry (RP-HPLC-QTOF/MS) and de novo sequencing tool to separate and identify different short chain peptides. Thirty-seven peptides were identified in the hydrolysed protein extracts from the three macroalgae, five of them being common in brown and red macroalgae. After checking against BIOPEP database, several sequenced peptides were found within longer peptides with potential antibacterial activity. Any of the identified peptides had previously been identified in macroalgae.
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