dc.contributor.author | Marina Alegre, María Luisa | |
dc.contributor.author | Sánchez López, Elena | |
dc.contributor.author | Lageveen-Kammeijer, G. | |
dc.contributor.author | Ramautar, R. | |
dc.contributor.author | Peters, D. | |
dc.contributor.author | Mayboroda, Oleg A. | |
dc.contributor.author | Crego Navazo, Antonio Luis | |
dc.date.accessioned | 2020-02-17T07:12:15Z | |
dc.date.available | 2020-02-17T07:12:15Z | |
dc.date.issued | 2019-01-28 | |
dc.identifier.bibliographicCitation | Scientific Reports, 2019, v. 9, n. 806, p. 1-9 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/10017/41066 | en |
dc.description.abstract | Capillary electrophoresis-mass spectrometry (CE-MS) using a sheathless porous tip interface emerged as an attractive tool in metabolomics thanks to its numerous advantages. One of the main advantages compared to the classical co-axial sheath liquid interface is the increased sensitivity, while maintaining the inherent properties of CE, such as a high separation efficiency and low sample consumption. Specially, the ability to perform nanoliter-based injections from only a few microliters of material in the sample vial makes sheathless CE-MS a well-suited and unique approach for highly sensitive metabolic profiling of limited sample amounts. Therefore, in this work, we demonstrate the utility of sheathless CE-MS for metabolic profiling of biomass-restricted samples, namely for 20 mu m-thick tissue sections of kidney from a mouse model of polycystic kidney disease (PKD). The extraction method was designed in such a way to keep a minimum sample-volume in the injection vial, thereby still allowing multiple nanoliter injections for repeatability studies. The developed strategy enabled to differentiate between different stages of PKD and as well changes in a variety of different metabolites could be annotated over experimental groups. These metabolites include carnitine, glutamine, creatine, betaine and creatinine. Overall, this study shows the utility of sheathless CE-MS for biomass-limited metabolomics studies. | en |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.rights | Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) | en |
dc.rights | © The Author(s) 2019 | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | en |
dc.title | Sheathless CE-MS based metabolic profiling of kidney tissue section samples from a mouse model of Polycystic Kidney Disease | en |
dc.type | info:eu-repo/semantics/article | en |
dc.subject.eciencia | Química | es_ES |
dc.subject.eciencia | Chemistry | en |
dc.contributor.affiliation | Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería Química | es_ES |
dc.date.updated | 2020-02-17T07:11:25Z | |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.identifier.doi | 10.1038/s41598-018-37512-8 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | en |
dc.identifier.uxxi | AR/0000030254 | en |
dc.identifier.publicationtitle | Scientific Reports | en |
dc.identifier.publicationvolume | 9 | |
dc.identifier.publicationlastpage | 9 | |
dc.identifier.publicationissue | 806 | |
dc.identifier.publicationfirstpage | 1 | |