RT info:eu-repo/semantics/article
T1 Novel strategy for the revalorization of olive (Olea europaea) residues based on the extraction of bioactive peptides
A1 Esteve Gil, Clara
A1 Marina Alegre, María Luisa
A1 García López, María Concepción
K1 Olive seeds
K1 Bioactive peptides
K1 Antioxidant capacity
K1 Antihypertensive capacity
K1 Mass spectrometry
K1 Química
K1 Chemistry
AB This work proposes a new strategy for the revalorization of residual materials from table-olive and olive oil production based on the extraction of bioactive peptides. Enzymatic hydrolysates of olive seed protein isolate were prepared by treatment with five different proteases: Alcalase, Thermolysin, Neutrase, Flavourzyme and PTN. Although all hydrolysates presented antioxidant properties, Alcalase was the enzyme that yielded the hydrolysate with the highest antioxidant capacity. All hydrolysates showed antihypertensive capacity, obtaining IC50 values from 29 to 350 mu g/ml. Thermolysin was the enzyme which yielded the hydrolysate with the highest ACE-inhibitory capacity. Hydrolysates were fractionated by ultrafiltration showing a high concentration of short chain peptides, which exhibited significantly higher antioxidant and antihypertensive capacities than fractions with higher molecular weights. Peptides in most active fractions were identified by LC-MS/MS, observing homologies with other recognized antioxidant and antihypertensive peptides. Finally, their antioxidant and antihypertensive capacities were evaluated after in vitro gastrointestinal digestion. (C) 2014 Elsevier Ltd. All rights reserved.
SN 0308-8146
YR 2015
FD 2015-01-15
LK http://hdl.handle.net/10017/48228
UL http://hdl.handle.net/10017/48228
LA eng
DS MINDS@UW
RD 02-may-2024