RT info:eu-repo/semantics/article
T1 Fluorescein labelled cationic carbosilane dendritic systems for biological studies
A1 Fuentes Paniagua, María Elena
A1 Serramia, M. Jesus
A1 Sánchez-Nieves Fernández, Javier
A1 Álvarez, Susana
A1 Muñoz Fernández, María Ángeles
A1 Gómez Ramírez, Rafael
A1 Mata de la Mata, Francisco Javier de la
K1 Labelled dendrimers and dendrons
K1 Carbosilane
K1 Gene therapy
K1 HIV
K1 Biodistribution
K1 Química
K1 Chemistry
AB Cationic carbosilane dendrimers and dendrons labelled with one fluorescein unit have been synthesized. For dendrimers (generations 1–3), a random procedure was followed by successive addition of two types of thiol compounds to vinyl terminated derivatives, first one with –NH3Cl and second one with –NMe2HCl functions, subsequent reaction with FITC and finally quaternization with MeI. For dendrons, the use of compounds with a –NH2 group at the focal point and –NMe2 functions at the periphery allowed us to obtain the corresponding fluoresceinated cationic derivatives. The toxicity of these dendritic molecules was studied by MTT and their interaction with siRNA Nef by electrophoresis. Finally, second generation dendrimer and their dendriplexes with siRNA Nef were chosen as a model to analyse their in vivo biodistribution in a BALB/c mouse model. The highest levels for dendriplexes were found in spleen and liver, followed in lymph nodes, while lower levels were found in kidneys. This distribution is in accordance with long circulation times.
SN 0014-3057
YR 2015
FD 2015-07-23
LK http://hdl.handle.net/10017/34022
UL http://hdl.handle.net/10017/34022
LA eng
NO Ministerio de Economía y Empresa
DS MINDS@UW
RD 28-abr-2024