RT info:eu-repo/semantics/article
T1 Minocycline provides protection against ß-amyloid(25-35)-induced alterations of the somatostatin signaling pathway in the rat temporal cortex
A1 Burgos Ramos, Emma
A1 Puebla Jiménez, Lilian
A1 Arilla Ferreiro, Eduardo
K1 Brain
K1 Alzheimer's disease
K1 Amyloid-ß
K1 CREB
K1 GRK
K1 Somatostatin receptors
K1 Bioquímica
K1 Biochemistry
K1 Science
K1 Ciencia
AB Minocycline is a semi-synthetic second-generation tetracycline known to improve cognition in amyloid precursor protein transgenic mice. Whether it can protect the somatostatin (SRIF) receptor-effector system, also involved in learning and memory, from alterations induced by chronic i.c.v. infusion of ß-amyloid peptide (Aß)(25-35) is presently unknown. Hence, in the present study, we tested the effects of minocycline on the SRIF signaling pathway in the rat temporal cortex. To this end, male Wistar rats were injected with minocycline (45 mg/kg body weight) i.p. twice on the first day of treatment. On the following day and during 14 days, Aß(25-35) was administered i.c.v. via an osmotic minipump connected to a cannula implanted in the left lateral ventricle (300 pmol/day). Minocycline (22.5 mg/kg, i.p.) was injected once again the last 2 days of the Aß(25-35) infusion. The animals were killed by decapitation 24 h after the last drug injection. Our results show that minocycline prevents the decrease in SRIF receptor density and somatostatin receptor (sst) 2 expression and the attenuated capacity of SRIF to inhibit adenylyl cyclase (AC) activity, alterations present in the temporal cortex of Aß(25-35)-treated rats. Furthermore, minocycline blocks the Aß(25-35)-induced decrease in phosphorylated cyclic AMP (cAMP) response element binding protein (p-CREB) content and G-protein-coupled receptor kinase 2 (GRK) protein expression in this brain area. Altogether, the present data demonstrate that minocycline in vivo provides protection against Aß-induced impairment of the SRIF signal transduction pathway in the rat temporal cortex and suggest that it may have a potential as a therapeutic agent in human Alzheimer's disease, although further studies are warranted.
PB Elsevier
SN 0306-4522
YR 2008
FD 2008
LK http://hdl.handle.net/10017/2362
UL http://hdl.handle.net/10017/2362
LA eng
NO Ministerio de Ciencia y Tecnología
DS MINDS@UW
RD 02-may-2024