RT info:eu-repo/semantics/article
T1 A sarabande of tropical fruit proteomics: Avocado, banana, and mango
A1 Marina Alegre, María Luisa
A1 Righetti, Pier Giorgio
A1 Esteve Gil, Clara
A1 D'Amato, Alfonsina
A1 Fasoli, Elisa
A1 García López, María Concepción
K1 Allergens
K1 Combinatorial peptide ligand libraries
K1 Low abundance protein MS
K1 Plant proteomics
K1 Tropical fruits
K1 Ciencia
K1 Química
K1 Science
K1 Chemistry
AB The present review highlights the progress made in plant proteomics via the introduction of combinatorial peptide ligand libraries (CPLL) for detecting low-abundance species. Thanks to a novel approach to the CPLL methodology, namely, that of performing the capture both under native and denaturing conditions, identifying plant species in the order of thousands, rather than hundreds, is now possible. We report here data on a trio of tropical fruits, namely, banana, avocado, and mango. The first two are classified as “recalcitrant” tissues since minute amounts of proteins (in the order of 1%) are embedded on a very large matrix of plant-specific material (e.g., polysaccharides and other plant polymers). Yet, even under these adverse conditions we could report, in a single sweep, from 1000 to 3000 unique gene products. In the case of mango the investigation has been extended to the peel too, since this skin is popularly used to flavor dishes in Far East cuisine. Even in this tough peel 330 proteins could be identified, whereas in soft peels, such as in lemons, one thousand unique species could be detected.
PB Wiley
SN 1615-9861
YR 2015
FD 2015
LK http://hdl.handle.net/10017/22938
UL http://hdl.handle.net/10017/22938
LA eng
DS MINDS@UW
RD 29-abr-2024