A novel controlled drug-delivery system for growth hormone applied to healing skin wounds in diabetic rats
Autores
Gimeno Longas, M. José; García Esteo, Francisco Javier; García Honduvilla, Natalio Antonio; San Roman, J.; Bellón Caneiro, Juan Manuel; [et al.]Identificadores
Enlace permanente (URI): http://hdl.handle.net/10017/6711DOI: 10.1163/156856203768366549
ISSN: 0920-5063
Editor
Brill Academic Publishers
Fecha de publicación
2003Cita bibliográfica
Journal of Biomaterials Science, Polymer Edition, 2003, v. 14, n. 8, p. 821-835
Palabras clave
Controlled release systems
Diabetes
Growth hormone
Skin
Wound healing
Proyectos
MAT98-0964-C02-02 (Comisión Interministerial de Ciencia y Tecnología-CICYT)
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Versión del editor
http://dx.doi.org/10.1163/156856203768366549Derechos
© EBSCO Publishing, 2003
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
Controlled release systems for drugs, hormones and growth factors can be particularly useful in tissue repair processes. These systems act as a biodegradable support containing the
substance to be delivered, allowing their gradual release. In the past years, the local application
of growth factors has acquired special relevance as a therapeutic option for use in subjects who show
de cient tissue scarring, the hormone dose being the limiting factor for its success. In this study, the in
vitro biocompatibility of a copolymer formed by vinylpyrrolidone and 2-hydroxyethyl methacrylate,
used as an administration vehicle for hGH, was evaluated. The system was then tested in vivo in
terms of its capacity for healing incisional wounds in healthy and diabetic rats. For the in vitro
studies, polymer and hormone degradation rates were determined, and polymer biocompatibility was
evaluated in broblast cultures. In the in vivo experiments, an incision was made in the back of the
animals, and polymers discs with/ without hGH, were introduced in the aperture. Morphological,
immunohistochemical and morphometric evaluations were performed on wound tissue specimens
3¿10 days after surgery. In vitro, the polymer was found to be biodegradable and showed no toxic
effects on broblasts, the hormone being slowly released to the culture medium. In untreated diabetic
rats, a delayed skin scarring and cell response were observed, compared to that noted in healthy
animals. Skin closure, keratinisationand brosis occurred earlier in the presence of the polymer-hGH
system. The use of this co-polymer as an administrationvehicle for hGH improves the wound scarring
process in the pathological setting of diabetes.
Ficheros en el ítem
Ficheros | Tamaño | Formato |
|
---|---|---|---|
2348.pdf | 491.2Kb |
|
Ficheros | Tamaño | Formato |
|
---|---|---|---|
2348.pdf | 491.2Kb |
|