Proteolytic activity in Caenorhabditis elegans: soluble and insoluble fractions
Authors
Giménez Pardo, Consuelo; Vázquez López, C.; Armas Serra, Cristina de; Rodríguez Caabeiro, Filomena CrisantaIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/61368DOI: 10.1017/S0022149X99000189
ISSN: 0022-149X
Date
1999-06Bibliographic citation
Journal of Helminthology, 1999, v. 73, n. 2, p. 123-127
Keywords
Caenorhabditis elegans
proteasas
fracciones solubles
fracciones insolubles
Description / Notes
Se realiza la deteccion de actividad proteolitica en fracciones solubles e insolubles del nematodo de vida libre Caenorhabditis elegans utilizando diferentes sustratos y temperaturas. Se ensayan tambien diferentes inhibidores dela actividad proteolitica bajo las mismas condiciones. Los resultados indican que la actividad proteolitica de ste nematodo aparece en ambas fracciones. Aparecen exo y endoproteasas tambien en las mismas fracciones.
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/aceptedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© CAB International
Access rights
info:eu-repo/semantics/openAccess
Abstract
Proteolytic activities of soluble and insoluble fractions of the free-living soil nematode Caenorhabditis elegans were measured across a range of substrates, temperatures and pH conditions. Several protease inhibitors were also tested under these conditions. Results of these studies indicate that proteolytic activity is present in cytosolic (CF) and non-cytosolic (NCF) fractions of C. elegans extracts at every condition of pH, temperature and buffer assayed. On the other hand, the use of different protease inhibitors demonstrated the existence of exo and endoproteases types in CF as well as NCF. Moreover our results show that the use of two protease inhibitor mixed types proposed by several authors are not enough to avoid this lytic activity when homogenates of this nematode are employed in biochemical assays.
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