Purification and partial characterization of proteinase activity in eggs of Dicrocoelium dendriticum
Identifiers
Permanent link (URI): http://hdl.handle.net/10017/61338DOI: 10.1079/joh200035
ISSN: 0022-149X
Date
2001-03Bibliographic citation
Journal of Helminthology, 2001, v. 75, n. 1, p. 57-59
Keywords
Purificación
Proteasas
Dicrocoelium dendriticum
Description / Notes
Se ha llevado a cabo una purificación preliminar mediante electroforesis de elución continua de una proteasa de 49,5 kDa de extractos crudos de huevos de Dicrocoelium dendriticum. La enzima mostró una alta capacidad para degradar el derivado del colágeno azocoll a pH ácido. Aunque es necesario realizar más experimentos para confirmar cualquier papel fisiológico, esta proteasa podría estar implicada en los mecanismos de penetración.
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/aceptedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© CAB International, 2001
Access rights
info:eu-repo/semantics/openAccess
Abstract
A preliminary purification has been carried out by continuous elution electrophoresis of a 49.5 kDa protease of crude extracts from Dicrocoelium dendriticum eggs. The enzyme showed a high capacity to degrade the collagen derivative azocoll at acidic pH. Although it is necessary to carry out further experiments to confirm any physiological role, this protease could be implicated in penetration mechanisms.
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Purification_Gimenez_JHelm_2001.pdf | 561.3Kb |
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Purification_Gimenez_JHelm_2001.pdf | 561.3Kb |
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