Antioxidants facilitate high-intensity exercise IL-15 expression in skeletal muscle
Authors
Pérez López, Alberto; Martín Rincón, Marcos; Santana, Alfredo; Pérez Suarez, Ismael; Dorado, Cecilia; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/60700DOI: 10.1055/a-0781-2527
ISSN: 0172-4622
Date
2019-01-01Affiliation
Universidad de Alcalá. Departamento de Ciencias Biomédicas. Unidad Docente de Educación Física y DeportivaFunders
Ministerio de Educación y Ciencia
Universidad de Las Palmas de Gran Canaria
Universidad Europea de Madrid
Universidad de Alcalá
Bibliographic citation
International Journal of Sports Medicine, 2019, v. 40, n. 1, p. 16-22
Keywords
Myokines
IL-15
Sprint exercise
Glycolysis
Oxidative stress
Description / Notes
10 p.
Project
info:eu-repo/grantAgreement/MEC//DEP2010-21866/ES//
info:eu-repo/grantAgreement/MEC//DEP2015-71171/ES//
info:eu-repo/grantAgreement/ULPGC//ULPGC 2015%05/ES//
info:eu-repo/grantAgreement/UEM//2010%01RM/ES//
info:eu-repo/grantAgreement/UAH//CCG2015%BIO-069/ES//
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/acceptedVersion
Rights
© Georg Thieme Verlag KG Stuttgart
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
Interleukin (IL)-15 stimulates mitochondrial biogenesis, fat oxidation, glucose uptake and myogenesis in skeletal muscle. However, the mechanisms by which exercise triggers IL-15 expression remain to be elucidated in humans. This study aimed at determining whether high-intensity exercise and exercise-induced RONS stimulate IL-15/IL-15R? expression and its signaling pathway (STAT3) in human skeletal muscle. Nine volunteers performed a 30-s Wingate test in normoxia and hypoxia (PIO2=75 mmHg), 2 h after placebo or antioxidant administration (?-lipoic acid, vitamin C and E) in a randomized double-blind design. Blood samples and muscle biopsies (vastus lateralis) were obtained before, immediately after, and 30 and 120 min post-exercise. Sprint exercise upregulated skeletal muscle IL-15 protein expression (ANOVA, P=0.05), an effect accentuated by antioxidant administration in hypoxia (ANOVA, P=0.022). In antioxidant conditions, the increased IL-15 expression at 120 min post-exercise (33%; P=0.017) was associated with the oxygen deficit caused by the sprint (r=-0.54; P=0.020); while, IL-15 and Tyr705-STAT3 AUCs were also related (r=0.50; P=0.036). Antioxidant administration promotes IL-15 protein expression in human skeletal muscle after sprint exercise, particularly in severe acute hypoxia. Therefore, during intense muscle contraction, a reduced PO2 and glycolytic rate, and possibly, an attenuated RONS generation may facilitate IL-15 production, accompanied by STAT3 activation, in a process that does not require AMPK phosphorylation.
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antioxidants_perez_ijsm_2019.pdf | 812.6Kb |
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