Mesothelial versus endothelial cell seeding: evaluation of cell adherence to a fibroblastic matrix using 111In oxine
Identifiers
Permanent link (URI): http://hdl.handle.net/10017/59624DOI: 10.1016/s1078-5884(97)80010-x
PMID: 9091146
ISSN: 1078-5884
Date
1997-02Affiliation
Universidad de Alcalá. Departamento Biomedicina y Biotecnología. Unidad docente Biología Celular y GenéticaBibliographic citation
Bellon, J.M. et al. (1997) ‘Mesothelial versus endothelial cell seeding: Evaluation of cell adherence to a fibroblastic matrix using In oxine’, European journal of vascular and endovascular surgery, 13(2), pp. 142–148. doi:10.1016/S1078-5884(97)80010-X.
Keywords
Endothelial cell seeding
Mesothelial cell seeding
Vascular prostheses
Document type
info:eu-repo/semantics/article
Rights
© 1997 W. B. Saunders Company Ltd.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Access rights
info:eu-repo/semantics/openAccess
Abstract
The aim of this study was to compare the behaviour of mesothelial cells (MC) to that of endothelial cells (EC) when seeded onto a PTFE, prosthesis coated with a fibroblastic matrix.
Three study groups were examined: a control group (Control) of PTFE prostheses with a fibroblast matrix (n = 8); Group EC, PTFE prostheses seeded with EC on a fibroblastic matrix (n = 8); and Group MC, PTFE, prostheses seeded with MC on a fibroblastic matrix (n = 8). All cell types were labelled with 111In (100 microCi/ml) 24 h after seeding, when the cells had formed a monolayer on the prosthetic surface. Radioactive levels were measured at 2, 4, 6, and 24 h.
Both EC and MC showed optimal adherence. The MC had a better radioactive uptake and retention than the EC. The number of EC and MC cells that remained adherent to the matrix was large enough to ensure complete covering of the prosthetic surface.
The use of MC is therefore feasible as an optimal alternative for achieving a natural covering on vascular prostheses prepared with a fibroblastic matrix.
Files in this item
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mesothelial_bellon_EJVES_1997.pdf | 3.207Mb |
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mesothelial_bellon_EJVES_1997.pdf | 3.207Mb |
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