Flow Cytometric análisis of the in situ hybridization of Cyclooxygenase (COX) isoforms in mesangial cells (MC) treated with cyclosporine A (CsA)
Authors
Pérez De Hornedo , J.; Calvino Fernández, Mirian; Arriba de la Fuente, Gabriel de; Del Reino Fernández , P; Parra Cid, Trinidad; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/59297DOI: 10.1002/cyto.a.20230
ISSN: 1552-4922
Date
2006Affiliation
Universidad de Alcalá. Departamento de MedicinaFunders
Junta de Comunidades de Castilla-La Mancha
Bibliographic citation
Cytometry Part A, 2006, v. 69a, n. , p. 161-164
Keywords
Cyclosporine
Cyclooxygenase
Oxidative stress
Reactive oxygen species
Flow cytometry
In situ hybridization
Description / Notes
4 p.
Project
info:eu-repo/grantAgreement/JCMM//0465/ES//
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution 4.0 International (CC BY 4.0)
Access rights
info:eu-repo/semantics/openAccess
Abstract
Background: Cyclosporine A increases oxidative stress in kidney and we hypothesized that cyclooxygenase (COX) may be involved in this effect. Material and Methods: Mesangial cells of Cyclosporine A-treated (4, 7 or 10 days) rats were obtained to evaluate mRNA expression of COX-isoforms (COX-1, constitutive and COX-2, inducible) by ??in situ?? hybridization. Probes were labelled using ??Gene Image Random Prime Labelling Protocol?? and COX expression was measured by flow cytometry. Results and Discussion: ??In situ?? hybridization by flow cytometry is an useful method to detect mRNA. We observed an increased COX-2 expression in a time-dependent manner in parallel with Reactive Oxygen Species synthesis. COX-1 expression increased only at 10 days.
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flow_hornedo_CYTOMETRY_2006.pdf | 100.6Kb |
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