Tropoelastin and fibulin overexpression in the subepitelial connective tissue of human pterigium
Authors
Bellón Caneiro, Juan Manuel; Pérez Rico, María Consuelo; Pascual González, María Gemma; Sotomayor Núñez, Sandra; Montes Mollón, Ma; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/59174DOI: 10.1016/j.ajo.2010.07.012
ISSN: 0002-9394
Date
2011-01-01Funders
Hospital Universitario Príncipe de Asturias (Alcalá de Henares, España)
Instituto de Salud Carlos III (Madrid, España).
Bibliographic citation
American Journal of Ophthalmology, 2011, v. 151 , n.1 , p. 44-52
Keywords
Calcium-Binding Proteins
Collagen Type I
Collagen Type III
Conjunctiva
Connective Tissue
Extracellular Matrix Proteins
Humans
Immunoenzyme Techniques
Middle Aged
Pterygium
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger
Tropoelastin
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© 2024 Elsevier B.V.
Access rights
info:eu-repo/semantics/openAccess
Abstract
Purpose To evaluate possible changes in the collagen and elastic components of the subepithelial connective tissue of human pterygium. Design Immunohistochemical study. Methods Immunohistochemical staining using antitropoelastin, anti-fibulin-2, and anti-fibulin-3 antibodies was performed in 10 normal conjunctival and 20 pterygium specimens. Masson trichome staining also was performed to study subepithelial connective tissue. Sirius red staining was used to identify collagen type I and III components. Tropoelastin, fibulin-2, and fibulin-3 messenger ribonucleic acid (mRNA) expressions were analyzed in 9 conjunctival and 12 pterygium specimens by quantitative real-time polymerase chain reaction assay. Results The subepithelial connective tissue and vessels were more predominant in pterygium compared with the normal conjunctival tissue. Amorphous subepithelial zones were observed in the areas of the pterygium tissue, but not in normal conjunctiva. Increased tropoelastin staining was seen in the pterygium tissue with areas of degenerative changes or immature formation of elastic fibers, as well an increase in tropoelastin mRNA, in contrast with fibulin-2 and fibulin-3 messenger levels. Fibulin-2 and fibulin-3 expression was colocalized in the subepithelial connective tissue and was distributed along blood and lymphatic vessels. Collagen type III, an immature form of collagen, was increased in the pathologic samples in association with a tissue remodeling process. Conclusions Elastin metabolism is dysregulated in the pathogenesis of human pterygium with tropoelastin, fibulin-2, and fibulin-3 overexpression in the subepithelial connective tissue.
Files in this item
Files | Size | Format |
|
---|---|---|---|
tropoelastin_perez_AJO_2011.pdf | 1.938Mb |
|
Files | Size | Format |
|
---|---|---|---|
tropoelastin_perez_AJO_2011.pdf | 1.938Mb |
|
Collections
- MEDICINA - Artículos [41]