A Practical Beginner's Guide to Raman microscopy
Authors
Montalvo García, Gemma; López Fernández, Adrián; Ortega Ojeda, Fernando Ernesto; García Ruiz, Carmen; Zapata Arráez, FélixIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/49371DOI: 10.1080/05704928.2020.1797761
ISSN: 0570-4928
Date
2021-06-16Embargo end date
2022-06-16Affiliation
Universidad de Alcalá. Departamento de Física y Matemáticas; Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaBibliographic citation
Applied Spectroscopy Reviews, 2021, v. 56, n. 6, p. 439-462
Keywords
Raman spectroscopy
Spectroscopy
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© Taylor & Francis Online, 2020
Access rights
info:eu-repo/semantics/openAccess
Abstract
Raman microscopy is a highly suitable and well settled down analytical techniquefor qualitative determination of chemical substances. However, many universityundergraduate chemical degrees do not incorporate its practical training in theexperimental laboratory practises that constitute their curricula. For this reason,this work aimed at designing a practical beginner's guide to Raman microscopyuseful for undergraduate students, teachers or practitioners who need to use it forthe first time. After a brief explanation of the main concepts about Ramanmicroscopy, the methodology development and results interpretation are mainlyexplained using paracetamol (acetaminophen) drugs as example. In addition, thisguide presents an application to the identification of different components withina mixture, which shows the instrumental potential and how to use it effectively.Finally, acetaminophen, ascorbic acid, and sucrose were positively detected usingRaman microscopy on a commercial drug whose major component wasacetaminophen. In fact, the guide shows the detection and unequivocalidentification of different components in the mixtures, even for those lowconcentration components (5-10 % mass ratio). This work clearly proposesdifferent pragmatic criteria at the laboratory for identifying substances in mixturesto promote an easy implementation of the Raman microscopy technique.
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