Enantiomeric separation of non-protein amino acids by Electrokinetic Chromatography
Identificadores
Enlace permanente (URI): http://hdl.handle.net/10017/48159DOI: 10.1016/j.chroma.2016.06.058
ISSN: 0021-9673
Fecha de publicación
2016-06-18Filiación
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaCita bibliográfica
Journal of Chromatography A, 2016, v. 1467, p. 409-416
Palabras clave
Non-protein amino acids
Enantiomeric separation
Chiral CE
Electrokinetic chromatography
Citrulline
Food supplements
Proyectos
info:eu-repo/grantAgreement/MINECO//CTQ2013-48740-P/ES/METODOLOGÍAS AVANZADAS PARA ANÁLISIS QUIRAL MEDIANTE TÉCNICAS MICROSEPARATIVAS. BÚSQUEDA DE BIOMARCADORES EN SISTEMAS ENANTIOSELECTIVOS
info:eu-repo/grantAgreement/MINECO//CTQ2013-48740-P/ES/METODOLOGIAS AVANZADAS PARA ANALISIS QUIRAL MEDIANTE TECNICAS MICROSEPARATIVAS. BUSQUEDA DE BIOMARCADORES EN SISTEMAS ENANTIOSELECTIVOS/
info:eu-repo/grantAgreement/CAM//S2013%2FABI-3028/ES/Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos/AVANSECAL-CM
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Derechos
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
© Elsevier
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
New analytical methodologies enabling the enantiomeric separation of a group of non-protein amino acids of interest in the pharmaceutical and food analysis fields were developed in this work using Electrokinetic Chromatography. The use of FMOC as derivatization reagent and the subsequent separation using acidic conditions (formate buffer at pH 2.0) and anionic cyclodextrins as chiral selectors allowed the chiral separation of eight from the ten non-protein amino acids studied. Pyroglutamic acid, norvaline, norleucine, 3,4-dihydroxyphenilalanine, 2-aminoadipic acid, and selenomethionine were enantiomericaly separated using sulfated-alpha-CD while sulfated-gamma-CD enabled the enantiomeric separation of norvaline, 3,4-dihydroxyphenilalanine, 2-aminoadipic acid, selenomethionie, citrulline, and pipecolic acid. Moreover, the potential of the developed methodologies was demonstrated in the analysis of citrulline and its enantiomeric impurity in food supplements. For that purpose, experimental and instrumental variables were optimized and the analytical characteristics of the proposed method were evaluated. LODs of 2.1 x 10(-7) and 1.8 x 10(-7) M for D-and L-citrulline, respectively, were obtained. D-Cit was not detectable in any of the six food supplement samples analyzed showing that the effect of storage time on the racemization of citrulline was negligible. (C) 2016 Elsevier B.V. All rights reserved.
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