In vitro antitumor and hypotensive activity of peptides from olive seeds
Autores
Vásquez Villanueva, Romy Ángela; Muñoz Moreno, Laura; Carmena Sierra, María José; Marina Alegre, María Luisa; García López, María ConcepciónIdentificadores
Enlace permanente (URI): http://hdl.handle.net/10017/48153DOI: 10.1016/j.jff.2017.12.062
ISSN: 1756-4646
Fecha de publicación
2018-03Filiación
Universidad de Alcalá. Departamento de Biología de Sistemas; Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaCita bibliográfica
Journal of Functional Foods, 2018, v. 42, p. 177-184
Palabras clave
Peptide
Olive seeds
Antitumor capacity
Antihypertensive capacity
Proyectos
info:eu-repo/grantAgreement/CAM//S2013%2FABI-3028/ES/Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos/AVANSECAL-CM
info:eu-repo/grantAgreement/MINECO//AGL2016-79010-R/ES/DESARROLLO DE METODOS SOSTENIBLES PARA EL APROVECHAMIENTO DE SUBPRODUCTOS DE LA INDUSTRIA ALIMENTARIA CON ELEVADO CONTENIDO PROTEICO
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Derechos
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
© Elsevier
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
Peptides with molecular weights below 3 kDa from the hydrolysis of olive seed proteins with Thermolysin (OS-3 kDa) have demonstrated a high in vitro hypotensive effect. This fraction has been further fractionated by semipreparative RP-HPLC to obtain 8 fractions. ACE inhibitor capacity of fractions was evaluated observing the highest capacity in fraction F5. Peptides in fraction F5 were identified by RP-HPLC- and HILIC-ESI-Q-ToF and cytotoxic effect was assessed in different cell lines. Peptide LLPSY, present in this faction, was synthesized and characterized. Despite antihypertensive capacity was not as high as in fraction F5, a significant anti-proliferative capacity on two different cancer cell lines was observed. Additional studies to assess antitumor activity confirmed that this peptide showed capability to increase the adhesion capacity of tumor cells, to decrease the migration capacity of cancer cells, and to arrest cell cycle on S phase.
Ficheros en el ítem
Ficheros | Tamaño | Formato |
|
---|---|---|---|
Invitro_Vasquez_JFunctFoods_20 ... | 1.256Mb |
|
Ficheros | Tamaño | Formato |
|
---|---|---|---|
Invitro_Vasquez_JFunctFoods_20 ... | 1.256Mb |
|
Colecciones
- QUANING - Artículos [367]