Isolation and identification by high resolution liquid chromatography tandem mass spectrometry of novel peptides with multifunctional lipid-lowering capacity
Identificadores
Enlace permanente (URI): http://hdl.handle.net/10017/48008DOI: 10.1016/j.foodres.2018.05.009
ISSN: 0963-9969
Fecha de publicación
2018-09Filiación
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaCita bibliográfica
Food Research International, 2018, v. 111, p. 77-86
Palabras clave
HPLC-MS/MS
Hypolipidemia
Peptides
Olive by-product
Proyectos
info:eu-repo/grantAgreement/CDTI//ITC-20151193/ES/DESARROLLO DE NUEVOS ALIMENTOS FUNCIONALES BASADOS EN LA INCORPORACIÓN DE PÉPTIDOS BIOACTIVOS EXTRAÍDOS A PARTIR DE SUBPRODUCTOS DE LA INDUSTRIA AGROALIMENTARIA: VALORIZACIÓN DE HUESOS DE FRUTAS Y ACEITUNAS
info:eu-repo/grantAgreement/MINECO//AGL2016-79010-R/ES/DESARROLLO DE METODOS SOSTENIBLES PARA EL APROVECHAMIENTO DE SUBPRODUCTOS DE LA INDUSTRIA ALIMENTARIA CON ELEVADO CONTENIDO PROTEICO
info:eu-repo/grantAgreement/CAM//S2013%2FABI-3028/ES/Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos/AVANSECAL-CM
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Derechos
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
© Elsevier
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
This work describes the isolation, characterization, and identification by RP-HPLC-ESI-Q-TOF of novel peptides that interfere in the fat digestion and absorption mechanisms by multiple pathways. Peptides were ultrafiltrated and peptides in the most active fraction were further separated by semipreparative RP-HPLC. Nine different subfractions were obtained observing a high amount of peptides in subfraction F3. Peptides in subfraction F3 could simultaneously reduce the solubility of cholesterol in micelles and inhibit pancreatic cholesterol esterase and pancreatic lipase, even after a simulated gastrointestinal digestion. The identification of lipid-lowering peptides has been scarcely performed and when done, low selectivity or sensitivity of employed identification techniques or conditions did not yield reliable results. Separation and detection of peptides by RP-HPLC-ESI-Q-TOF-MS was optimized and most favorable conditions were employed for the identification of peptides using de novo sequencing. Ten different peptides with 4-9 amino acids were identified. Main feature of identified peptides was the high acidity derived from a high presence of amino acids glutamic acid and aspartic acid in their sequences.
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