Gold nanorods as SERS substrate for the ultratrace detection of cocaine in non-pretreated oral fluid samples
Autores
D'Elia, Valentina; Rubio Retama, Benito Jorge; Ortega Ojeda, Fernando Ernesto; García Ruiz, Carmen; Montalvo García, GemmaIdentificadores
Enlace permanente (URI): http://hdl.handle.net/10017/46567DOI: 10.1016/j.colsurfa.2018.05.068
ISSN: 0927-7757
Fecha de publicación
2018-11-20Filiación
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaCita bibliográfica
Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2018, v. 557, p. 43-50
Palabras clave
Cocaine
nanoparticles
gold nanorods
oral fluid
surface enhanced Raman spectroscopy
orthogonal partial least squares to latent structures-discriminate analysis (OPLS-DA)
Proyectos
CCG2015/EXP-028 (Universidad de Alcalá)
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Derechos
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© Elsevier, 2018
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
This work shows the capability of surface-enhanced Raman spectroscopy (SERS) to detect ultratraces of cocaine(COC) in oral fluid (OF). It proposes a new solid substrate made of gold nanorods (Au NRs) to perform sensitiveanalyses of this complex matrix without any sample pretreatment. The Au NRs were synthetized optimizing thesynthesis conditions, and then characterized and tested as SERS substrate. The presented results demonstratedthat the SERS methodology was able to detect COC in OF with a limit of detection (LOD) as low as 10 ng/mL.This value was five orders of magnitude smaller than the one obtained with Raman spectroscopy (RS) and in theorder of the cut-off value (8 ng/mL) established for confirmatory test of COC in OF. Besides, a multivariateanalysis (OPLS-DA) applied on the samples analysed by SERS evidenced that it was possible to discriminateamong various COC concentrations. These are quite positive results because even the 1 ng/mL COC concentration sample could be identified as different from the OF sample. In addition, the absence of samplepretreatment and the ease of the proposed method, allowed performing rapid and non-destructive analyses,making the method suitable for in situ forensic analyses.
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