Probing the confinement of beta-galactosidase into meso-macro porous silica by Raman spectroscopy
Authors
Fontaínhas Prazeres, Sofía; Zapata Arráez, Félix; Canilho, Nadia; Pasc, Andreea; García Ruiz, Carmen; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/46547DOI: 10.1016/j.micromeso.2018.11.032
ISSN: 1387-1811
Date
2019-04Embargo end date
2021-05-01Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaBibliographic citation
Microporous and Mesoporous Materials, 2019, v. 278, p. 149-155
Keywords
beta-galactosidase
Biocatalysts
Meso-macroporous silica
Enzyme-support interactions
Raman spectroscopy
Project
FP7-PEOPLE-2013-ITN-606713 (Comisión Europea "Marie Curie Initial Training Networks (Multi-Partner ITN))
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© Elsevier, 2019
Access rights
info:eu-repo/semantics/openAccess
Abstract
Immobilization of beta-galactosidase (beta-gal) into porous materials might afford to supported biocatalysts for the hydrolysis of diary products or to food additives for lactose intolerant people. Activity and stability of the loaded material generally depend on the interactions between the enzyme and the support, as well as on the pore size. Herein, Raman spectroscopy was used to evaluate the specific adsorption the enzyme into meso-macroporous silica materials, containing interconnected mesopores of 9 nm and macropores of 200 nm. Non-porous silica was used as reference material to determine the Raman fingerprint of physisorbed enzyme in the absence of any confinement. While the beta-gal physisorbed on the surface of non-porous silica material exhibits the same Raman spectra as the free enzyme, the enzyme physisorbed onto meso-macroporous materials show frequency displacements of characteristic amide groups as a function of initial concentration of the feed enzymatic solution. In fact, at low initial concentration in enzyme, no shifts of the amides were recorded on Raman spectra as compared with free enzyme, indicating a preferential physisorption into macropores. By increasing the enzyme concentration, the frequency of Amide I was shifted to lower values, suggesting thus a confinement into mesopores. Finally, the enzyme concentration effect can be demonstrated by the increment of the amide band intensity in the range of 1700-1500 cm(-1) as the amount of adsorbed enzyme increases. Thus, the textural properties of silica materials seem to be the key factor in the enzyme adsorption.
Files in this item
Files | Size | Format |
|
---|---|---|---|
Probing_Prazeres_MicroporMesop ... | 3.873Mb |
|
Files | Size | Format |
|
---|---|---|---|
Probing_Prazeres_MicroporMesop ... | 3.873Mb |
|
Collections
- QUANING - Artículos [367]