Multi-target methodology for the screening of blood specimens in drug-facilitated sexual assault cases
Identifiers
Permanent link (URI): http://hdl.handle.net/10017/46107DOI: doi.org/10.1016/j.microc.2019.104204
ISSN: 0026-265X
Date
2019-11Embargo end date
2021-11-30Affiliation
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaBibliographic citation
Microchemical Journal, 2019, v. 150, n. 104204
Keywords
Drug facilitated sexual assault (DFSA)
Drug facilitated crime (DFC)
Blood
Protein precipitation
Phospholipid removal
Multi-target screening
Project
DGPNSD-133548-2018I032 (Ministerio de Sanidad, Consumo y Bienestar Social - Delegación del Gobierno para el Plan Nacional sobre Drogas)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© Elsevier, 2019
Access rights
info:eu-repo/semantics/openAccess
Abstract
The forensic investigation of drug-facilitated sexual assaults (DFSA) requires the toxicological examination of biological matrices to detect possible substances in victims. Whole blood is an important sample since it is theonly one available for analysis in a considerable percentage of cases. A new screening methodology was developed for the detection of 96 compounds related to DFSA in blood. Sample treatment was based on a procedurecombining protein precipitation and phospholipid removal before HPLC-MS/MS analysis. The new method operates in scheduled multiple reaction monitoring (sMRM) mode and uses the hybrid operating of the thirdquadrupole as an ion trap. The blood samples preparation was studied comparing four different protein precipitation treatments and two widely used extraction techniques as solid phase extraction and liquid-liquid extraction. The analytical performance of the screening method was evaluated in terms of selectivity and limits of detection. Finally, successful results were obtained applying the new method to the analysis of fifteen samples from real cases of the National Institute of Toxicology and Forensic Sciences.
Files in this item
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Multi_Prego_MicrochemJ_2019.pdf | 1.128Mb |
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Files | Size | Format |
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Multi_Prego_MicrochemJ_2019.pdf | 1.128Mb |
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