Nanomaterials in protein sample preparation
Identificadores
Enlace permanente (URI): http://hdl.handle.net/10017/44567DOI: 10.1080/15422119.2019.1581216
ISSN: 1542-2119
Fecha de publicación
2020-07-02Fecha fin de embargo
2021-07-03Filiación
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaCita bibliográfica
Separation and Purification Reviews, 2020, v. 49, n. 3, p. 229-264
Palabras clave
Protein sample preparation
nanomaterials
extraction
enrichment
purification
digestion
enzyme
Proyectos
info:eu-repo/grantAgreement/CAM//S2018%2FBAA-4393/ES/ESTRATEGIAS INTEGRADAS PARA LA MEJORA DE LA CALIDAD, LA SEGURIDAD Y LA FUNCIONALIDAD DE LOS ALIMENTOS: HACIA UNA ALIMENTACIÓN SALUDABLE/AVANSECAL-II
info:eu-repo/grantAgreement/MINECO//AGL2016-79010-R/ES/DESARROLLO DE METODOS SOSTENIBLES PARA EL APROVECHAMIENTO DE SUBPRODUCTOS DE LA INDUSTRIA ALIMENTARIA CON ELEVADO CONTENIDO PROTEICO
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Derechos
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© Taylor & Francis
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
Protein sample preparation is the most critical step in protein analysis of complex samples and is constituted by tedious, time-consuming, and difficult-to-automate steps that usually involve the use of high volumes of solvents. In recent years, novel extraction or digestion nanomaterials (NMs) have been developed aiming to overcome these drawbacks. In this review, we have grouped the recent works related to the development of new NMs and their applications to the extraction, enrichment/purification, and digestion of proteins. This paper evaluates the role of different kinds of NMs in each step of protein sample preparation focusing on the type of established interaction between the protein and the nanomaterial, their sensitivity and selectivity, their adsorption capacity, and the advantages that they bring in relation to time, efficiency, or reusability.
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