Analysis of human bodily fluids on superabsorbent pads by ATR-FTIR
Identificadores
Enlace permanente (URI): http://hdl.handle.net/10017/36206DOI: 10.1016/j.talanta.2016.10.061
ISSN: 0039-9140
Fecha de publicación
2017-01-01Filiación
Universidad de Alcalá. Departamento de Química Analítica, Química Física e Ingeniería QuímicaPatrocinadores
Instituto Universitario de Investigación en ciencias policiales
Universidad de Alcalá
Cita bibliográfica
Talanta, 2017, v. 162, p. 634-640
Palabras clave
ATR-FTIR
Semen
Urine
Vaginal fluid
Mixtures of bodily fluids
Superabsorbent pads
Proyectos
IUICP-2016-002 (Instituto Universitario de Investigación en ciencias policiales - Universidad de Alcalá)
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Derechos
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
© Elsevier, 2017
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
Superabsorbent pads are composed of different layers with different grades of absorbent capacity, being the lower one the most absorbent layer. Due to their complexity, the analysis of bodily fluids on superabsorbent pads is certainly difficult. In this study, semen, vaginal fluid and urine stains placed on superabsorbent pads including sanitary napkins, panty-liners and diapers were non-destructively detected by Attenuated Total Reflectance (ATR) Fourier Transform Infrared spectroscopy (FTIR). In spite of the higher absorbent capacity of the lower layers, this technique was able to detect the three fluids on the upper layer of all pads, showing that bodily fluids are distributed within all layers. Additionally, mixtures of these bodily fluids prepared on superabsorbent pads and cotton were studied, since real forensic investigations involving sexual abuse cases usually deal with mixtures of these fluids. Due to their IR marked protein region (1800-1480 cm-1), semen and vaginal fluid were easily distinguished from urine. However, since semen and vaginal fluid have both a high protein composition, that region of their IR signatures were quite similar, except for slight visual differences, that should be further analysed. Therefore, we propose ATR-FTIR as a suitable, presumptive, non-destructive and rapid approach to detect stains of human bodily fluids on the upper layer of superabsorbent pads from sexual crimes.
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