Fluorescein labelled cationic carbosilane dendritic systems for biological studies
Authors
Fuentes Paniagua, María Elena; Serramia, M. Jesus; Sánchez-Nieves Fernández, Javier; Álvarez, Susana; Muñoz Fernández, María Ángeles; [et al.]Identifiers
Permanent link (URI): http://hdl.handle.net/10017/34022DOI: 10.1016/j.eurpolymj.2015.07.043
ISSN: 0014-3057
Date
2015-07-23Funders
Ministerio de Economía y Empresa
Comunidad de Madrid
Ministerio de Educación y Ciencia
Bibliographic citation
European Polymer Journal, 2015, v. 71, p. 61-72
Keywords
Labelled dendrimers and dendrons
Carbosilane
Gene therapy
HIV
Biodistribution
Project
info:eu-repo/grantAgreement/MINECO//CTQ2011-23245/ES/DISEÑO DE NANOSISTEMAS DENDRITICOS BIFUNCIONALES DE NATURALEZA CARBOSILANO PARA SU APLICACIÓN EN BIOMEDICINA
info:eu-repo/grantAgreement/MINECO//CTQ-2014-54004-P/ES/Diseño de sistemas dendríticos avanzados para su uso en biomedicina obtenidos a través de procesos de dendronización
info:eu-repo/grantAgreement/CAM//S2011%2FBMD-2351/ES/Nanosistemas Dendríticos para Aplicaciones Biomédicas - Dendritic Nanosystems for Biomedical Aplications
info:eu-repo/grantAgreement/CAM//S2011%2FBMD-2351/ES/Nanosistemas Dendríticos para Aplicaciones Biomédicas - Dendritic Nanosystems for Biomedical Aplications
info:eu-repo/grantAgreement/CAM//S-2010%2FBMD-2332/ES/Redes moleculares y celulares en enfermedades inflamatorias/INDISNET
info:eu-repo/grantAgreement/MEC//AP2010-1470/ES
info:eu-repo/grantAgreement/UE//PT13%2F0010%2F0028/UE/Plataforma de Biobancos
info:eu-repo/grantAgreement/ISCIII//PI13%2F02016/ES/Desarrollo y mecanismo de acción de dendrímeros como microbicidas para frenar la infección por el VIH por transmisión sexual (vaginal y anal): prueba de concepto
info:eu-repo/grantAgreement/MINTUR//RD12%2F0017%2F0037/ES/SUBPROGRAMA RETICS (Redes Temáticas de Investigación Cooperativa en Salud): Red de SIDA (RIS)
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/acceptedVersion
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)(c) Elsevier, 2015
(c) Elsevier, 2015
Access rights
info:eu-repo/semantics/openAccess
Abstract
Cationic carbosilane dendrimers and dendrons labelled with one fluorescein unit have been synthesized. For dendrimers (generations 1–3), a random procedure was followed by successive addition of two types of thiol compounds to vinyl terminated derivatives, first one with –NH3Cl and second one with –NMe2HCl functions, subsequent reaction with FITC and finally quaternization with MeI. For dendrons, the use of compounds with a –NH2 group at the focal point and –NMe2 functions at the periphery allowed us to obtain the corresponding fluoresceinated cationic derivatives. The toxicity of these dendritic molecules was studied by MTT and their interaction with siRNA Nef by electrophoresis. Finally, second generation dendrimer and their dendriplexes with siRNA Nef were chosen as a model to analyse their in vivo biodistribution in a BALB/c mouse model. The highest levels for dendriplexes were found in spleen and liver, followed in lymph nodes, while lower levels were found in kidneys. This distribution is in accordance with long circulation times.
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fluorescein_gomez_european_2015.pdf | 3.344Mb |
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fluorescein_gomez_european_2015.pdf | 3.344Mb |
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