Mesoporous Silica Nanoparticles Decorated with Carbosilane Dendrons as New Non-viral Oligonucleotide Delivery Carriers
Autores
Gómez Ramírez, Rafael; Martínez Martínez, Ángel; Fuentes Paniagua, María Elena; Baeza García, Alejandro; Mata de la Mata, Francisco Javier de la; [et al.]Identificadores
Enlace permanente (URI): http://hdl.handle.net/10017/33947DOI: 10.1002/chem.201501966
ISSN: 0947-6539
Editor
Wiley
Fecha de publicación
2015-09-10Patrocinadores
Ministerio de Economía y Empresa
Comunidad de Madrid
Cita bibliográfica
Chemistry - A European Journal, 2015, v. 21, p. 1-17
Palabras clave
Carbosilane dendrons
Mesoporous silica nanoparticles
Gene transfection
Nonviral vectors
Proyectos
info:eu-repo/grantAgreement/MINECO//MAT2012-35556/ES/BIOCERÁMICAS DE SÍLICE MESOPOROSA CON PROPIEDADES ESTÍMULO-RESPUESTA PARA EL TRATAMIENTO SECUENCIAL DE PATOLOGÍAS DEL TEJIDO ÓSEO
info:eu-repo/grantAgreement/CAM//S2011%2FBMD-2351/ES/Nanosistemas Dendríticos para Aplicaciones Biomédicas - Dendritic Nanosystems for Biomedical Aplications
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/acceptedVersion
Derechos
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
(c) Wiley, 2015
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
A novel nanosystem based on mesoporous silica nanoparticles covered with carbosilane dendrons grafted on their external surface is reported. This system is able to transport single oligonucleotide strands into cells, avoiding the electrostatic repulsion between the cell membrane and the negatively charged nucleic acids thanks to the cationic charge provided by the dendron coating in physiological conditions. Moreover, the presence of the highly ordered pore network inside the silica matrix would make possible to allocate other therapeutic agents within the mesopores with the aim of achieving a double delivery. First, carbosilane dendrons of second and third generation pos- sessing ammonium or tertiary amine groups as peripheral functional groups were prepared. Hence, different strategies were tested in order to obtain their suitable grafting on the nanoparticles outer surface. As nucleic acid model, a single stranded DNA oligonucleotide tagged with a fluorescent Cy3 moiety was used to evaluate the DNA adsorption capacity. The hybrid material functionalized with the third generation of neutral dendron showed excellent DNA binding properties. Finally, the cytotoxicity as well as the capability to deliver DNA into cells, was tested using a human osteoblast-like cell line, achieving good levels of internalization of the vector DNA/carbosilane dendron functionalized material without affecting cellular viability.
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