Effect of cysteamine on cytosolic somatostatin binding-sites in rabbit duodenal mucosa
Autores
González Guijarro, Luis A.; López Ruiz, María del Pilar; Bodega Magro, Guillermo; Prieto Villapún, Juan Carlos; Arilla Ferreiro, EduardoIdentificadores
Enlace permanente (URI): http://hdl.handle.net/10017/2472DOI: 10.1016/0014-4800(87)90061-X
ISSN: 0014-4800
Editor
Academic Press
Fecha de publicación
1987Patrocinadores
Instituto de Salud Carlos III
Cita bibliográfica
Experimental and molecular pathology, 1987, v. 46, n. 2, p. 153-158
Palabras clave
Somatostatin
Rabbit
Mucosa
Proyectos
info:eu-repo/grantAgreement/ISCIII//85%2F1213/ES//
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Versión del editor
http://dx.doi.org/10.1016/0014-4800(87)90061-XDerechos
(c) Academic Press, 1987
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
Administration of cysteamine in rabbits elicited a rapid depletion of both duodenal mucosa and plasma somatostatin. A significant reduction was observed within 5 min, returning toward control values by 150 min. The depletion of somatostatin was associated with an increase in the binding capacity and a decrease in the affinity of both high- and low-affinity binding sites present in cytosol of duodenal mucosa. Incubation of cytosolic fraction from control rabbits with 1 mM cysteamine did not modify somatostatin binding. Furthermore, addition of cysteamine at the time of binding assay did not affect the integrity of 125I-Tyr11-somatostatin. It is concluded that in vivo administration of cysteamine to rabbits depletes both duodenal mucosa and plasma somatostatin and leads to up-regulation of duodenal somatostatin binding sites.
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