Identification by hydrophilic interaction and reversed-phase liquid chromatography-tandem mass spectrometry of peptides with antioxidant capacity in food residues
Identificadores
Enlace permanente (URI): http://hdl.handle.net/10017/24608DOI: 10.1016/j.chroma.2015.07.032
ISSN: 0021-9673
Editor
Elsevier
Fecha de publicación
2016Fecha fin de embargo
2017-07-12Cita bibliográfica
Journal of Chromatography A, 2016, v.1428, n.8, p.185-192
Palabras clave
Hydrophilic interaction
Reversed-phase
Liquid chromatography–tandem mass spectrometry
Peptides
Antioxidant
Peach seed
Proyectos
info:eu-repo/grantAgreement/MINECO//AGL2012-36362/ES/VALORIZACION DE RESIDUOS SOLIDOS VEGETALES PROCEDENTES DE LA INDUSTRIA AGROALIMENTARIA/
info:eu-repo/grantAgreement/CAM//S2013%2FABI-3028/Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos/AVANSECAL-CM
info:eu-repo/grantAgreement/UAH//CCG2013%2FEXP-028//ES
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/acceptedVersion
Versión del editor
http://dx.doi.org/10.1016/j.chroma.2015.07.032Derechos
Atribución-NoComercial-SinDerivadas 3.0 España
© Elsevier, 2015
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
HILIC- and RP-HPLC-ESI-Q-TOF identification of bioactive peptides with antioxidant capacity in peach by-products was carried out. Peach seeds contain more than 40% of proteins (as dried and defatted basis) and could constitute a cheap source of bioactive peptides. Extracted proteins were digested using four different commercial enzymes. Five assays based on different antioxidant mechanisms were employed for a reliable evaluation of the antioxidant capacity of the extracts. Thermolysin enzyme originated the extract with the most favorable antioxidant capacity. Probably due to a synergic effect among antioxidant peptides, it was not possible to find a peptide fraction with a higher antioxidant capacity than the whole extract. Eighteen peptides were identified in the whole hydrolysate when combining HILIC- and RP-HPLC-ESI-Q-TOF. A high percentage of hydrophobic amino acids were observed within their sequences which is a characteristic feature of the antioxidant nature of peptides.
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