Revalorization of a peach (Prunus persica (L.) Batsch) byproduct: Extraction and characterization of ACE-inhibitory peptides from peach stones
Identificadores
Enlace permanente (URI): http://hdl.handle.net/10017/24604DOI: 10.1016/j.jff.2015.06.056
ISSN: 1756-4646
Editor
Elsevier
Fecha de publicación
2015Fecha fin de embargo
2016-07-25Cita bibliográfica
Journal of Functional Foods, 2015, v.18, n.A, p.137-146
Palabras clave
Peach
Seed
Peptides
ACE-inhibitor
De novo sequencing
Proyectos
info:eu-repo/grantAgreement/MINECO//AGL2012-36362/ES/VALORIZACION DE RESIDUOS SOLIDOS VEGETALES PROCEDENTES DE LA INDUSTRIA AGROALIMENTARIA/
info:eu-repo/grantAgreement/UAH//CCG2013%2FEXP-028//ES
info:eu-repo/grantAgreement/CAM//S2013%2FABI-3028/Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos/AVANSECAL-CM
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/acceptedVersion
Versión del editor
http://dx.doi.org/10.1016/j.jff.2015.06.056Derechos
Atribución-NoComercial-SinDerivadas 3.0 España
© Elsevier, 2015
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
The value-added use of peach stone for production of a high protein product was investigated. Different parameters were optimized in order to obtain the highest protein yield in the extraction of peach seed proteins. Optimal conditions enabled the extraction of 43 g of protein per 100 g of dried and defatted milled seeds. Different enzymes and parameters were also tried in order to obtain the highest hydrolysis degree in the digestion of this peach seed protein isolate. Thermolysin was the enzyme that yielded the extract with the highest ACE-inhibition capability. After ultrafiltration through different molecular weight cut-off filters, potential antihypertensive peptides remained in the fraction below 3 kDa. Three different peptides (LYSPH, LYTPH, and HLLP) resisting simulated gastrointestinal digestion and ACE activity and demonstrating antihypertensive in vitro capacity were identified by HPLC-MS/MS. This is the first time that potential antihypertensive peptides have been isolated from peach stones.
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Revalorization_peach_JOFF_2015.pdf | 496.6Kb |
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Revalorization_peach_JOFF_2015.pdf | 496.6Kb |
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