Fractionation and identification of antioxidant and angiotensin-converting enzyme-inhibitory peptides obtained from plum (Prunus domestica L.) stones
Autores
Marina Alegre, María Luisa; González García, Estefanía; Puchalska, Patrycja Anna; García López, María ConcepciónIdentificadores
Enlace permanente (URI): http://hdl.handle.net/10017/24159DOI: 10.1016/j.jff.2015.08.033
ISSN: 1756-4646
Editor
Elsevier
Fecha de publicación
2015Fecha fin de embargo
2016-12-01Patrocinadores
Novozymes Spain S.A.
Cita bibliográfica
Journal of Functional Foods, 2015, v.19, n.A, p.376–384
Palabras clave
Plum stone
ACE-inhibitory peptides
Antioxidant peptides
Hydrophilic interaction chromatography
Mass spectrometry
Proyectos
info:eu-repo/grantAgreement/MINECO//AGL2012-36362/ES/VALORIZACION DE RESIDUOS SOLIDOS VEGETALES PROCEDENTES DE LA INDUSTRIA AGROALIMENTARIA
info:eu-repo/grantAgreement/UAH//CCG2013%2FEXP-028//ES
info:eu-repo/grantAgreement/CAM//S2013/ABI-3028/ES/Estrategias avanzadas para la mejora y el control de la calidad y la seguridad de los alimentos/AVANSECAL-CM
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/acceptedVersion
Versión del editor
http://dx.doi.org/10.1016/j.jff.2015.08.033Derechos
© Elsevier, 2015
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
Peptides with antioxidant and ACE-inhibitory capacities have been obtained from a plum processing by-product. The aim of this work was to fractionate most active plum hydrolysates, to identify peptides in these fractions, and to evaluate their resistance to gastrointestinal enzymes. Highest antioxidant capacity was observed in the whole Alcalase hydrolysate while peptides yielding the highest ability to inhibit ACE were concentrated in the fraction <3 kDa from the Thermolysin hydrolysate. RP-HPLC–ESI-MS/MS enabled the identification of 13 and 7 potential antioxidant and ACE inhibitory peptides, respectively, in these fractions. Nevertheless, RP-HPLC alone did not guarantee the successful separation of all peptides. The combination of RP-HPLC and hydrophilic interaction liquid chromatography (HILIC) coupled to ESI-Q-TOF-MS/MS enabled the identification of all peptides. Five potential antioxidant and three potential antihypertensive peptides could be additionally identified by HILIC. A total of 7 potential antioxidant peptides and 3 potential antihypertensive peptides resisted a simulated gastrointestinal digestion.
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