Response of rat cerebral somatostatinergic system to a high ammonia diet
Identificadores
Enlace permanente (URI): http://hdl.handle.net/10017/2274DOI: 10.1016/0197-0186(96)00021-6
ISSN: 0197-0186
Editor
Pergamon
Fecha de publicación
1996Patrocinadores
Universidad de Alcalá
Instituto de Salud Carlos III
Ministerio de Educación y Cultura
Cita bibliográfica
Neurochemistry International, 1996, v. 29, n. 5, p. 469–476
Palabras clave
Encephalopathy
Wistar rats
Ammonium acetate
Somatostatin
Frontoparietal cortex
Hippocampus
Somatostatin
Proyectos
info:eu-repo/grantAgreement/UAH//001%2F96/ES/REGULACION DEL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA POR EL NEUROPEPTIDO Y (NPY) EN EL CEREBRO DE LA RATA
info:eu-repo/grantAgreement/UAH//008%2F96/ES/MODULACION DEL SISTEMA SOMATOSTATINÉRGICO CEREBRAL DE LA RATA POR EL ÓXIDO NÍTRICO
info:eu-repo/grantAgreement/MEC//PM95-0041/ES/EFECTO DEL OXIDO NITRICO SOBRE EL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA EN EL CEREBRO HUMANO Y DE RATA
info:eu-repo/grantAgreement/ISCIII//94%2F0401/ES//
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Versión del editor
http://dx.doi.org/10.1016/0197-0186(96)00021-6Derechos
© Elsevier Science Ltd, 1996
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
It has been reported that ingestion of an ammonium-containing diet produces hyperammonemia without encephalopathy, thus permitting the study of the specific effects of ammonia toxicity. The present study investigated the rat cerebral somatostatinergic system using this experimental model of hyperammonemia. Wistar rats were fed a high ammonia diet prepared by mixing a standard diet with ammonium acetate (20% w/w); in addition, 5 mM of ammonium acetate was added to their water supply. Control rats were fed with a standard diet. The animals were sacrificed at 3, 7 and 15 days of ammonia ingestion. Ammonia levels in blood had increased ¿3-fold at 7 days of ammonia ingestion. These changes were associated with a significant decrease in the specific binding of somatostatin (SS) to putative receptors sites in the frontoparietal cortex and hippocampus at 7 and 15 days after starting the high ammonia diet. Scatchard analysis shows that the decrease in SS binding resulted from a decrease in the number of available SS receptors rather than a change in receptor affinity. No changes in the somatostatin-like immunoreactivity content (SSLI) were detected in either brain area at the three study times. These results suggest that hyperammonemia alone can affect the rat brain somatostatinergic system. However, the animal model of hyperammonemia used here is insufficient to produce encephalopathy despite the significant increase in serum ammonia.
Ficheros en el ítem
Ficheros | Tamaño | Formato |
|
---|---|---|---|
RESPONSE OF RAT.pdf | 637.2Kb |
|
Ficheros | Tamaño | Formato |
|
---|---|---|---|
RESPONSE OF RAT.pdf | 637.2Kb |
|
Colecciones
- BIOQBM - Artículos [137]