Effect of nitric oxide on the somatostatinergic system in the rat exocrine pancreas
Authors
Rodríguez Martín, Eulalia; Muñoz Acedo, Gema; Puebla Jiménez, Lilian; Arilla Ferreiro, EduardoIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/2175DOI: 10.1016/S0167-4889(99)00032-4
ISSN: 0006-3002
Publisher
Elsevier
Date
1999Funders
Ministerio de Educación y Cultura
Bibliographic citation
Biochimica et Biophysica Acta, 1999, v. 1450, n. 1, p. 61-67
Keywords
Nitric oxide
Somatostatin receptor
Adenylyl cyclase
Somatostatin-like immunoreactivity
Pancreatic acinar membrane
Rat
Project
info:eu-repo/grantAgreement/MEC//PM95-0041/ES/EFECTO DEL OXIDO NITRICO SOBRE EL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA EN EL CEREBRO HUMANO Y DE RATA
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Publisher's version
http://dx.doi.org/10.1016/S0167-4889(99)00032-4Rights
(c) Elsevier, 1999
Access rights
info:eu-repo/semantics/openAccess
Abstract
Nitric oxide (NO) and somatostatin (SS) are two important mediators of the exocrine and endocrine pancreas, exerting opposite effects on this organ. There is strong evidence suggesting an interaction between pancreatic NO and SS. The aim of this study was to determine whether L-arginine (L-Arg), the substrate for NO synthase (NOS), and Nω-nitro-L-arginine methyl ester (L-NAME), a NOS inhibitor, regulate pancreatic somatostatin-like immunoreactivity (SSLI) content and the SS mechanism of action in pancreatic acinar cell membranes. L-Arg (150 mg/kg, intraperitoneally (i.p.)), L-NAME (50 mg/kg, i.p.) or L-NAME plus L-Arg were injected twice daily at 8 h intervals for 8 days. L-Arg decreased pancreatic SSLI content as well as the number of SS receptors in pancreatic acinar cell membranes whereas L-NAME increased both parameters. The stable SS analogue SMS 201-995 induced a significantly lower inhibition of forskolin-stimulated adenylyl cyclase activity in pancreatic acinar cell membranes from L-Arg-treated rats whereas an increased inhibition was observed in pancreatic acinar membranes from L-NAME-treated rats. These results indicate that the NO system may contribute to the regulation of the pancreatic somatostatinergic system.
Files in this item
Files | Size | Format |
|
---|---|---|---|
articulo.pdf | 179.2Kb |
|
Files | Size | Format |
|
---|---|---|---|
articulo.pdf | 179.2Kb |
|
Collections
- BIOQBM - Artículos [137]