Determination of betaines in vegetable oils by capillary electrophoresis tandem mass spectrometry. Application to the detection of olive oil adulteration with seed oils.
Autores
Sánchez Hernández, Laura; Castro Puyana, María; Marina Alegre, María Luisa; Crego Navazo, Antonio LuisIdentificadores
Enlace permanente (URI): http://hdl.handle.net/10017/21130DOI: 10.1002/elps.201100005
ISSN: 0173-0835
Editor
Wiley-VCH
Fecha de publicación
2011Patrocinadores
Authors thank the Spanish Ministry of Science and Innovation (project CTQ2009- 09022) and the Comunidad Autónoma of Madrid (Spain) and european funding from FEDER programme (project S2009/AGR-1464, ANALISYC-II). They also thank the University of Alcalá and the Comunidad Autónoma of Madrid for project CCG10- UAH/AGR-5950. Laura Sánchez-Hernández thanks the Comunidad Autónoma of Madrid for her research contract.
Cita bibliográfica
Electrophoresis, 2011, v. 32, n. 11 , p. 1394-1401
Palabras clave
Betaines
Capillary electrophoresis
Corn oil
Olive oil
Soybean oil
Sunflower oil
Tandem mass spectrometry
Descripción
The final publication is available at Springer via http://dx.doi.org/10.1002/elps.201100005
Proyectos
info:eu-repo/grantAgreement/CAM//CCG10-UAH/AGR-5950/ES/METODOLOGÍAS ANALÍTICAS AVANZADAS POR ESPECTROMETRÍA DE MASAS ACOPLADA O NO A TÉCNICAS SEPARATIVAS EN FASE LÍQUIDA (LC/CE) PARA LA DETERMINACIÓN DE PEQUEÑAS Y GRANDES MOLÉCULAS EN EL CONTROL DE LA CALIDAD DE LOS ALIMENTOS
info:eu-repo/grantAgreement/MICIN//CTQ2009-09022//ES/NUEVAS ESTRATEGIAS ANALÍTICAS PARA LA DETERMINACIÓN DE ENANTIOMEROS Y PEPTIDOS BIOACTIVOS POR MICRO/NANO TÉCNICAS
info:eu-repo/grantAgreement/CAM//S2009/AGR-1464/ES/METODOLOGÍAS ANALÍTICAS INNOVADORAS PARA EL CONTROL DE LA CALIDAD Y LA SEGURIDAD DE LOS ALIMENTOS/ANALISYC-II
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/acceptedVersion
Versión del editor
http://dx.doi.org/10.1002/elps.201100005Derechos
(c) Wiley-VCH, 2011
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
A capillary electrophoresis-tandem mass spectrometry methodology enabling the
simultaneous determination of betaines (glycine betaine, trigonelline, proline betaine and
total content of carnitines) in vegetable oils was developed. Betaines were derivatized
with butanol previous to their baseline separation in 10 min using a 0.1 M formic acid
buffer at pH 2.0. Ion trap conditions were optimized in order to maximize selectivity and
sensitivity. Analytical characteristics of the proposed method were established by
evaluating its selectivity, linearity, precision (RSDs ranged from 4.8% to 10.7% for
corrected peak areas), accuracy by means of recovery studies (from 80% to 99%) and
LODs and LOQs at 0.1 ppb level. The method was applied to the determination of the
selected betaines in seed oils and extra virgin olive oils. MS2 experiments provided the
fingerprint fragmentation for all betaines studied in seed oils. In extra virgin olive oils,
carnitines were not detected being possible to propose them as a feasible novel marker for
the detection of adulterations of olive oils. Application of the developed method to the
analysis of different mixtures of extra virgin olive oil with seed oil (between 2-10 %)
enabled the detection and quantitation of the total content of carnitines. The results
obtained show the high potential of the developed method for the authentication and
quality control of olive oils.
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