Characterization and quantitation of soybean proteins in commercial soybean products by capillary electrophoresis
Authors
García Ruiz, Carmen; García López, María Concepción; Torre Roldán, María Mercedes; Marina Alegre, María LuisaIdentifiers
Permanent link (URI): http://hdl.handle.net/10017/1362DOI: 10.1002/(SICI)1522-2683(19990701)20:10<2003::AID-ELPS2003>3.0.CO;2-1
ISSN: 0173-0835
Publisher
John Wiley & Sons
Date
1999Funders
Universidad de Alcalá
Comunidad de Madrid
Bibliographic citation
Electrophoresis, 1999, v. 20, p. 2003-2012
Keywords
Capillary electrophoresis
Soybean proteins
Quantitation
Project
info:eu-repo/grantAgreement/UAH//E029%2F98/ES//
info:eu-repo/grantAgreement/CAM//COR 0035%2F94/ES//
info:eu-repo/grantAgreement/CAM//06G%2F047%2F96/ES//
Document type
info:eu-repo/semantics/article
Version
info:eu-repo/semantics/publishedVersion
Publisher's version
http://dx.doi.org/10.1002/(SICI)1522-2683(19990701)20:10<2003::AID-ELPS2003>3.0.CO;2-1Rights
(c) WILEY-VCH Verlag GmbH, 69451 Weinheim, 1999
Access rights
info:eu-repo/semantics/openAccess
Abstract
Capillary electrophoresis was applied for the first time to determine soybean proteins
in commercial soybean products. The most suitable conditions for the analysis of these
products in less than 7 min were 0.05 M phosphate buffer (pH 8) with 1 M urea; detection
wavelength, 254 nm; applied voltage, 20 kV; and temperature, 30o
C. Quantitation
of soybean proteins was achieved using referenced conditions by means of the method
of standard additions, using as standard a soybean protein isolate. This method
was validated and applied to the quantitation of soybean proteins in commercial products
derived from soybean protein isolate and soybean seeds.
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Soybean-Electrophoresis, 20, ... | 182.8Kb |
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Files | Size | Format |
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Soybean-Electrophoresis, 20, ... | 182.8Kb |
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