Rapid determination of salbutamol in pharmaceutical preparations by chiral capillary electrophoresis
Identificadores
Enlace permanente (URI): http://hdl.handle.net/10017/1359DOI: 10.1002/elps.200305490
ISSN: 0173-0835
Editor
John Wiley & Sons
Fecha de publicación
2003Patrocinadores
Comisión Interministerial de Ciencia y Tecnología
Universidad de Alcalá
Cita bibliográfica
Electrophoresis, 2003, v. 24, p. 2680-2686
Palabras clave
Chiral capillary electrophoresis
Pharmaceutical preparation
Salbutamol
Proyectos
info:eu-repo/grantAgreement/CICYT//PB98-0709/ES//
info:eu-repo/grantAgreement/UAH//E027%2F2001/ES/SEPARACIÓN ENANTIOMÉRICA DE COMPUESTOS QUIRALES POR ELECTROCROMATOGRAFÍA CAPILAR
Tipo de documento
info:eu-repo/semantics/article
Versión
info:eu-repo/semantics/publishedVersion
Versión del editor
http://dx.doi.org/10.1002/elps.200305490Derechos
© Wiley, 2003
Derechos de acceso
info:eu-repo/semantics/openAccess
Resumen
A fast and simple method of chiral capillary electrophoresis (CE) has been applied to the analysis of salbutamol in different pharmaceutical preparations. Using of a 25 mM acetate buffer (pH 5.0), containing 13.1 mg/mL carboxymethyl- -cyclodextrin (CM- -
CD), an applied voltage of 20 kV and a temperature of 25 C, the enantiomers of salbutamol could be separated in about 2 min. Three different pharmaceutical preparations (two syrups, one oral solution, and two kind of tablets) containing a racemate of salbutamol
were injected directly in the CE system, following dilution in dimethyl sulfoxide
(DMSO). Appreciable differences in the retention times were observed for salbutamol enantiomers in the different formulations studied, which were attributed to the effect of the matrix components on the electrophoretic mobility. The standard addition method
was used for the calibration due to the existence of matrix interferences. Finally, the stability of the enantiomers of salbutamol in the oral solution was studied calculating the enantiomeric ratio values when the solution was injected immediately after being opened in the first case and after being opened and stored in the fridge for two months in the second case.
Ficheros en el ítem
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Salbutamol-Electrophoresis, 24, ... | 87.91Kb |
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Salbutamol-Electrophoresis, 24, ... | 87.91Kb |
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