%0 Journal Article 
%A Martínez Girón, Ana Belén
%A García Ruiz, Carmen
%A Crego Navazo, Antonio Luis
%A Marina Alegre, María Luisa
%T Development of an in-capillary derivatization method by CE for the determination of chiral amino acids in dietary supplements and wines
%D 2009
%@ 0173-0835
%U http://hdl.handle.net/10017/3888
%X A fast in-capillary derivatization method by CE with 6-aminoquinolyl-N-hydroxysuccinimidyl
carbamate was developed for the first time for the determination of
amino acid enantiomers (arginine, lysine, and ornithine) in dietary supplements and
wines. Because of the initial current problems due to the formation of precipitates into
the capillary during the derivatization reaction, a washing step with an organic solvent as
DMSO between injections was necessary. Different approaches were also investigated to
enhance the sensitivity of detection. A derivatization procedure, where plugs of ACN,
derivatizing agent (10mM 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate), and
sample in borate (1:1 v/v) were injected in tandem (2, 3, and 6 s, respectively, at 50 mbar),
was selected because it enabled to obtain the most sensitive and reproducible results.
Appropriate analytical characteristics (linearity, LOD and LOQ, precision, absence of
matrix interferences, and accuracy) were obtained for this method. Finally, the optimized
method was successfully applied to the determination of the enantiomers of arginine,
lysine, and ornithine in food samples of different complexities (dietary supplements and
wines).
%K Amino acid
%K 6-Aminoquinolyl-N-hydroxysuccinimidyl carbamate
%K CE
%K Chiral method
%K In-capillary derivatization
%K Ciencia
%K Química analítica e industrial
%K Science
%K Chemistry, analytic and technical
%~ Biblioteca Universidad de Alcala