RT info:eu-repo/semantics/article
T1 Human pregnane X receptor is expressed in breast carcinomas, potential heterodimers formation between hPXR and RXR-alpha.
A1 Conde Martín, María Isabel
A1 Toledo Lobo, María del Val
A1 Zamora Romero, Javier
A1 Pérez Márquez, Julio
A1 Gonzalez, Francisco J.
A1 Alba Conejo, Emilio
A1 Fraile Laiz, Benito
A1 Paniagua Gómez-Álvarez, Ricardo
A1 Arenas Jiménez, María Isabel
K1 Human pregnane X receptor
K1 Breast cancer
K1 Biología
K1 Biology
K1 Genética
K1 Genetics
K1 Ciencia
K1 Science
AB The human pregnane X receptor (hPXR) is an orphan nuclear receptor that induces transcription of response elements present in steroid-inducible cytochrome P 450 gene promoters. This activation requires the participation of retinoid X receptors (RXRs), needed partners of hPXR to form heterodimers. We have investigated the expression of hPXR and RXRs in normal, premalignant, and malignant breast tissues, in order to det. whether their expression profile in localized infiltrative breast cancer is assocd. with an increased risk of recurrent disease. Methods: Breast samples from 99 patients including benign breast diseases, in situ and infiltrative carcinomas were processed for immunohistochem. and Western-blot anal. Results: Cancer cells from patients that developed recurrent disease showed a high cytoplasmic location of both hPXR isoforms. Only the infiltrative carcinomas that relapsed before 48 mo showed nuclear location of hPXR isoform 2. This location was assocd. with the nuclear immunoexpression of RXR-alpha. Conclusion: Breast cancer cells can express both variants 1 and 2 of hPXR. Infiltrative carcinomas that recurred showed a nuclear location of both hPXR and RXR-alpha; therefore, the overexpression and the subcellular location changes of hPXR could be considered as a potential new prognostic indicator.
PB BioMed Central
SN 1471-2407
YR 2008
FD 2008
LK http://hdl.handle.net/10017/5604
UL http://hdl.handle.net/10017/5604
LA eng
DS MINDS@UW
RD 19-abr-2024