RT info:eu-repo/semantics/article T1 Magnetic nanoparticles coated with carboxylate-terminated carbosilane dendrons as a reusable and green approach to extract/purify proteins A1 Prados Nieto, Isabel María A1 Sánchez-Nieves Fernández, Javier A1 Mata de la Mata, Francisco Javier de la A1 Marina Alegre, María Luisa A1 García López, María Concepción K1 Carbosilane dendron magnetic nanoparticles K1 ProteinSustainability K1 Purification K1 Extraction K1 Química K1 Chemistry AB Extraction/purification of proteins, at both analytical and industrial levels, is a limiting step that usually requires the use of organic solvents and involves tedious work and a high cost. This work proposes a more sustainable alternative based on the use of magnetic nanoparticles (MNPs) coated with carboxylate-terminated carbosilane dendrons. MNPs coated with first- and second-generation carbosilane dendrons and bare MNPs were employed for the extraction of proteins with different molecular weights and charges. Interaction of proteins with MNPs significantly varied with the pH, the protein, and the dendron generation (different sizes and number of charges in the periphery). Optimal dendron:protein molar ratios and suitable conditions for disrupting interactions after protein extraction were also researched. Second-generation dendron-coated MNPs showed 100% retention capability for all proteins when using acidic conditions. They were reused without losing magnetism or interaction capacity after a disruption of protein-dendron interactions with 0.2% SDS at 100 degrees C for 10 min. The capacity of dendron-coated MNPs was successfully applied to the recovery/purification of proteins from two food byproducts, olive seeds and cheese whey. SN 1618-2642 YR 2021 FD 2021-12-09 LK http://hdl.handle.net/10017/52231 UL http://hdl.handle.net/10017/52231 LA eng DS MINDS@UW RD 29-mar-2024