RT info:eu-repo/semantics/article
T1 Exploratory Metabolomic Analysis Based on Reversed-Phase Liquid Chromatography-Mass Spectrometry to Study an In Vitro Model of Hypoxia-Induced Metabolic Alterations in HK-2 Cells
A1 Bernardo Bermejo, Samuel
A1 Sánchez López, Elena
A1 Tan, Lei
A1 Benito Martínez, Selma
A1 Jiang, Z.
A1 Castro Puyana, María
A1 Lucio Cazaña, Francisco Javier de
A1 Marina Alegre, María Luisa
K1 HK-2 cells
K1 hypoxialiquid chromatography-mass spectrometry
K1 untargeted metabolomics
K1 multivariate analysis
K1 Química
K1 Chemistry
AB Oxygen deficiency in cells, tissues, and organs can not only prevent the proper development of biological functions but it can also lead to several diseases and disorders. In this sense, the kidney deserves special attention since hypoxia can be considered an important factor in the pathophysiology of both acute kidney injury and chronic kidney disease. To provide better knowledge to unveil the molecular mechanisms involved, new studies are necessary. In this sense, this work aims to study, for the first time, an in vitro model of hypoxia-induced metabolic alterations in human proximal tubular HK-2 cells because renal proximal tubules are particularly susceptible to hypoxia. Different groups of cells, cultivated under control and hypoxia conditions at 0.5, 5, 24, and 48 h, were investigated using untargeted metabolomic approaches based on reversed-phase liquid chromatography-mass spectrometry. Both intracellular and extracellular fluids were studied to obtain a large metabolite coverage. On the other hand, multivariate and univariate analyses were carried out to find the differences among the cell groups and to select the most relevant variables. The molecular features identified as affected metabolites were mainly amino acids and Amadori compounds. Insights about their biological relevance are also provided.
SN 1422-0067
YR 2021
FD 2021-07
LK http://hdl.handle.net/10017/52210
UL http://hdl.handle.net/10017/52210
LA eng
DS MINDS@UW
RD 27-abr-2024