RT info:eu-repo/semantics/article T1 Solution structure and stability of a disulfide cross-linked nucleopeptide duplex A1 Gómez Pinto, Irene A1 Marchan Sancho, Vicente A1 Gago Badenas, Federico A1 Grandas, Anna A1 González, Carlos K1 Ciencia K1 Farmacología K1 Science K1 Pharmacology AB NMR methods are used to study the structure and stability of the duplex formed by the nucleopeptide [Ac- Cys- Gly- Ala-Hse( p(3')dGCATGC)- Ala-OH](2)[S-S], in which the oligonucleotide is self-complementary and the cysteine residues of the two peptide chains form a disulfide bridge; thermal transitions and NMR-derived structural calculations are consistent with a 3-D structure in which the oligonucleotide forms a standard B-DNA helix without significant distortions; the peptide chains are relatively disordered in solution and lie in the minor groove of the DNA helix; this nucleopeptide duplex exhibits a high melting temperature, indicating that peptide - oligonucleotide conjugates containing cysteines are suitable molecules to establish cross-links between DNA strands and stabilize the duplex. PB Royal Society of Chemistry YR 2003 FD 2003 LK http://hdl.handle.net/10017/5123 UL http://hdl.handle.net/10017/5123 LA eng DS MINDS@UW RD 29-mar-2024