RT info:eu-repo/semantics/article
T1 Bisphenol A impaired cell adhesion by altering the expression of adhesion and cytoskeleton proteins on human podocytes
A1 Moreno Gómez-Toledano, Rafael
A1 Arenas Jiménez, María Isabel
A1 González Martínez, Clara
A1 Olea Herrero, Nuria
A1 Reventun Torralba, Paula
A1 Di Nunzio, Michele
A1 Sánchez Esteban, Sandra
A1 Arilla Ferreiro, Eduardo
A1 Saura Redondo, Marta
A1 Bosch Martínez, Ricardo José
K1 Biomedicina
K1 Biology
K1 Medicina
K1 Medicine
AB Bisphenol A (BPA), a chemical -xenoestrogen- used in food containers is present in the urine of almost the entire population. Recently, several extensive population studies have proven a significant association between urinary excretion of BPA and albuminuria. The alteration of glomerular podocytes or "podocytopathy" is a common event in chronic albuminuric conditions. Since many podocytes recovered from patients' urine are viable, we hypothesized that BPA could impair podocyte adhesion capabilities. Using an in vitro adhesion assay, we observed that BPA impaired podocyte adhesion, an effect that was abrogated by Tamoxifen (an estrogen receptor blocker). Genomic and proteomic analyses revealed that BPA affected the expression of several podocyte cytoskeleton and adhesion proteins. Western blot and immunocytochemistry confirmed the alteration in the protein expression of tubulin, vimentin, podocin, cofilin-1, vinculin, E-cadherin, nephrin, VCAM-1, tenascin-C, and β-catenin. Moreover, we also found that BPA, while decreased podocyte nitric oxide production, it lead to overproduction of ion superoxide. In conclusion, our data show that BPA induced a novel type of podocytopathy characterizes by an impairment of podocyte adhesion, by altering the expression of adhesion and cytoskeleton proteins. Moreover, BPA diminished production of podocyte nitric oxide and induced the overproduction of oxygen-free metabolites. These data provide a mechanism by which BPA could participate in the pathogenesis and progression of renal diseases.
SN 2045-2322
YR 2020
FD 2020-07-15
LK http://hdl.handle.net/10017/50400
UL http://hdl.handle.net/10017/50400
LA eng
DS MINDS@UW
RD 28-abr-2024